Preliminary comparison of precursor scans and liquid chromatography-tandem mass spectrometry on a hybrid quadrupole time-of-flight mass spectrometer. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Preliminary comparison of precursor scans and liquid chromatography-tandem mass spectrometry on a hybrid quadrupole time-of-flight mass spectrometer.

J Chromatogr A. 1999 Aug 27;854(1-2):119-30. Unique Identifier : AIDSLINE MED/99426221
Borchers C; Parker CE; Deterding LJ; Tomer KB; Laboratory of Structural Biology, National Institute of; Environmental Health Sciences, National Institutes of Health,; Research Triangle Park, NC 27709, USA.

Abstract: Recent mass spectrometry instrumentation developments include the appearance of novel hybrid tandem instrumentation, Q-TOF, consisting of a quadrupole mass analyzer (MS1) and a time-of-flight (TOF) analyzer. The TOF analyzer is not scanned, but collects all fragment ions entering the analyzer at a given time. Thus, the typical precursor scan experiment cannot be performed. Instead, a full MS-MS spectrum can be acquired for each mass passed by MS1. Appropriate data manipulation, i.e. extracted ion current chromatograms, can correlate specific fragment ion formation to the parent ion. Precursor scanning and LC-MS-MS are compared on a Q-TOF instrument for the determination of protein modifications, including acetylation and phosphorylation. Model peptides used for phosphopeptide detection were generated from a mixture of beta-casein. Model acetylated peptides were generated from a mixture of acetylated substance P1-9 and substance P1-11. The results were then applied to a more complex mixture, a digest of HIV-p24. Results indicate that precursor scanning is useful for screening, but that LC-MS-MS has a sensitivity advantage and is less susceptible to suppression effects. LC-MS-MS, therefore, appears to be better for the detection of trace components in complex mixtures.

Keywords: JOURNAL ARTICLE Acetylation Amino Acid Sequence Caseins/CHEMISTRY Chromatography, Liquid/*METHODS Comparative Study HIV Core Protein p24/CHEMISTRY Peptide Mapping Sensitivity and Specificity Spectrum Analysis, Mass/*METHODS Substance P/CHEMISTRY Support, Non-U.S. Gov't

KWDjournalarticleacetylationaminoacidsequencecaseins/chemistrychromatography,liquid/KWDmethodscomparativestudyhivcoreproteinp24/chemistrypeptidemappingsensitivityandspecificityspectrumanalysis,mass/KWDmethodssubstancep/chemistrysupport,non-uKWDsKWDgov't
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