Induction of a germinal center phenotype in B cells in vitro by a Th2 cell line. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Induction of a germinal center phenotype in B cells in vitro by a Th2 cell line.

Cell Immunol. 1999 Dec 15;198(2):77-86. Unique Identifier : AIDSLINE MED/20115380
Morafo V; Rath S; Thomas ML; Durdik JM; Department of Biological Sciences, University of Arkansas,; Fayetteville, Arkansas 72701, USA.

Abstract: We have investigated the contribution of various stimuli for generating in vitro the changes in surface phenotype characteristic of B cells responding to a T-dependent antigen in a germinal center (GC). We show that, unlike many other stimuli such as B cell mitogens, cytokines, and surrogate antigen, alone or in combination, an alloreactive Th2 clonal line induces splenic B cells to become cell surface peanut agglutinin (PNA)(hi), Ig(lo), CD62L(lo), and CD44(hi) to produce mRNA for M17 and to express a GC-specific transgene even without B cell receptor ligation. Neither proliferation nor prior activation of responding B cells is needed, but B cells from CD45-null mice show reduced efficiency of this induction. These findings open up possibilities for separation and dissection of the various components of the GC response. Copyright 1999 Academic Press.

Keywords: JOURNAL ARTICLE Animal Antigens, CD40/PHYSIOLOGY Antigens, CD45/PHYSIOLOGY B-Lymphocytes/*PHYSIOLOGY Cell Line Coculture Cytokines/PHARMACOLOGY Germinal Center/*PHYSIOLOGY *Lymphocyte Cooperation Lymphocyte Transformation Mice Mice, Inbred C57BL Phenotype Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th2 Cells/*PHYSIOLOGY

KWDjournalarticleanimalantigens,cd40/physiologyantigens,cd45/physiologyb-lymphocytes/KWDphysiologycelllinecoculturecytokines/pharmacologygerminalcenter/KWDphysiologyKWDlymphocytecooperationlymphocytetransformationmicemice,inbredc57blphenotypesupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDth2cells/KWDphysiology
000630
A0061970

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