Human vascular smooth muscle cells possess functional CCR5.

Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

Human vascular smooth muscle cells possess functional CCR5.

J Biol Chem. 2000 Feb 25;275(8):5466-71. Unique Identifier : AIDSLINE MED/20148745
Schecter AD; Calderon TM; Berman AB; McManus CM; Fallon JT; Rossikhina M; Zhao W; Christ G; Berman JW; Taubman MB; Zena and Michael A. Wiener Cardiovascular Institute, The Mount; Sinai School of Medicine, New York, New York 10029, USA.

Abstract: CC chemokine receptors are important modulators of inflammation. Although CC chemokine receptors have been found predominantly on leukocytes, recent studies have suggested that vascular smooth muscle cells respond to CC chemokines. We now report that human smooth muscle cells express CCR5, a co-receptor for human immunodeficiency virus. CCR5 mRNA was detectable by RNA blot hybridization in human aortic and coronary artery smooth muscle cells. The cDNA generated by reverse transcription-polymerase chain reaction from aortic smooth muscle cells had 100% identity throughout the entire coding region with the CCR5 cloned from THP-1 cells. By immunohistochemistry, CCR5 and the CCR5 ligand, macrophage inflammatory protein-1beta (MIP-1beta), were detected in smooth muscle cells and macrophages of the atherosclerotic plaque. In smooth muscle cell culture, MIP-1beta induced a significant increase in intracellular calcium concentrations, which was blocked by an antibody to CCR5. In addition, MIP-1beta caused a calcium-dependent increase in tissue factor activity. Tissue factor is the initiator of coagulation and is thought to play a key role in arterial thrombosis. These data suggest that human arterial smooth muscle cells express functional CCR5 receptors and MIP-1beta is an agonist for these cells.

Keywords: JOURNAL ARTICLE Aorta/METABOLISM Atherosclerosis/METABOLISM Calcium/METABOLISM Chelating Agents/PHARMACOLOGY Coronary Vessels/METABOLISM Dose-Response Relationship, Drug Egtazic Acid/ANALOGS & DERIVATIVES/PHARMACOLOGY Endothelium, Vascular/METABOLISM Human Immunohistochemistry Inflammation/METABOLISM Macrophage Inflammatory Protein-1/METABOLISM Muscle, Smooth, Vascular/*METABOLISM Receptors, CCR5/GENETICS/*METABOLISM Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger/METABOLISM Support, U.S. Gov't, P.H.S. Thromboplastin/METABOLISM Thrombosis/METABOLISM Time Factors Umbilical Cord/METABOLISM

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