Induction of diabetes in nonobese diabetic mice by Th2 T cell clones from a TCR transgenic mouse. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Induction of diabetes in nonobese diabetic mice by Th2 T cell clones from a TCR transgenic mouse.

J Immunol. 2000 Mar 15;164(6):3072-8. Unique Identifier : AIDSLINE MED/20171506
Poulin M; Haskins K; Department of Immunology and Barbara Davis Center for Childhood; Diabetes, University of Colorado Health Sciences Center, Denver,; CO 80262, USA.

Abstract: We have produced a panel of cloned T cell lines from the BDC-2.5 TCR transgenic (Tg) mouse that exhibit a Th2 cytokine phenotype in vitro but are highly diabetogenic in vivo. Unlike an earlier report in which T cells obtained from the Tg mouse were cultured for 1 wk under Th2-promoting conditions and were found to induce disease only in NOD.scid recipients, we found that long-term T cell clones with a fixed Th2 cytokine profile can transfer disease only to young nonobese diabetic (NOD) mice and never to NOD.scid recipients. Furthermore, the mechanism by which diabetes is transferred by a Tg Th2 T cell clone differs from that of the original CD4+ Th1 BDC-2.5 T cell clone made in this laboratory. Whereas the BDC-2.5 clone rapidly causes disease in NOD.scid recipients less than 2 wk old, the Tg Th2 T cell clones can do so only when cotransferred with other diabetogenic T cells, suggesting that the Th2 T cell requires the presence of host T cells for initiation of disease.

Keywords: JOURNAL ARTICLE Adoptive Transfer Animal Cell Line Clone Cells Diabetes Mellitus, Insulin-Dependent/ETIOLOGY/*GENETICS/ *IMMUNOLOGY/PATHOLOGY Female Lymphocyte Transfusion Mice Mice, Inbred NOD Mice, SCID Mice, Transgenic Receptors, Antigen, T-Cell, alpha-beta/*GENETICS Species Specificity Spleen/CYTOLOGY/TRANSPLANTATION Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th1 Cells/IMMUNOLOGY/TRANSPLANTATION Th2 Cells/IMMUNOLOGY/*TRANSPLANTATION

KWDjournalarticleadoptivetransferanimalcelllineclonecellsdiabetesmellitus,insulin-dependent/etiology/KWDgenetics/KWDimmunology/pathologyfemalelymphocytetransfusionmicemice,inbrednodmice,scidmice,transgenicreceptors,antigen,t-cell,alpha-beta/KWDgeneticsspeciesspecificityspleen/cytology/transplantationsupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDth1cells/immunology/transplantationth2cells/immunology/KWDtransplantation
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A0061912

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