Gene trap insertional mutagenesis in mice: new vectors and germ line mutations in two novel genes. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Gene trap insertional mutagenesis in mice: new vectors and germ line mutations in two novel genes.

Transgenic Res. 1999;8(6):451-8. Unique Identifier : AIDSLINE GENBANK/AF064783
Neilan EG; Barsh GS; Department of Pediatrics, Stanford University, Palo Alto, CA; 94305, USA.

Abstract: Insertional mutagenesis based on gene trap vectors that capture endogenous splice sites is a promising tool for functional genomics. Several groups have proposed large-scale gene trap screens, but questions remain as to the type of vectors and their design. We report a set of plasmid-encoded gene trap vectors and the disruption of two novel genes. Our results include a comparison of the relative gene trapping efficiencies of two different splice acceptor sequences in ES cells and an analysis of the structure of several gene trap insertions.

Keywords: JOURNAL ARTICLE beta-Galactosidase/GENETICS/METABOLISM Animal Cloning, Molecular Comparative Study Female Gene Expression Regulation Gene Products, env/GENETICS *Genetic Vectors *Germ-Line Mutation Male Mice Mice, Inbred Strains Mice, Mutant Strains Mice, Transgenic Molecular Sequence Data Moloney Leukemia Virus/GENETICS Mutagenesis, Insertional/*METHODS Phenotype RNA Splicing Support, Non-U.S. Gov'tKWDjournalarticlebeta-galactosidase/genetics/metabolismanimalcloning,molecularcomparativestudyfemalegeneexpressionregulationgeneproducts,env/geneticsKWDgeneticvectorsKWDgerm-linemutationmalemicemice,inbredstrainsmice,mutantstrainsmice,transgenicmolecularsequencedatamoloneyleukemiavirus/geneticsmutagenesis,insertional/KWDmethodsphenotypernasplicingsupport,non-uKWDsKWDgov't
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