Th2 T cells expressing transgene PDGF-A serve as vectors for gene therapy in autoimmune demyelinating disease. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Th2 T cells expressing transgene PDGF-A serve as vectors for gene therapy in autoimmune demyelinating disease.

J Autoimmun. 1999 Aug;13(1):31-8. Unique Identifier : AIDSLINE MED/99371722
Mathisen PM; Yu M; Yin L; Johnson JM; Kawczak JA; Nishiyama A; Tuohy VK; Department of Immunology, Lerner Research Institute, The; Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH,; 44195, USA.

Abstract: We hypothesized that T cells can be genetically modified to express growth factor transgene products capable of inducing oligodendrocyte progenitor proliferation. Autoreactive T cells isolated from SWXJ mice immunized with the p139-151 determinant of myelin proteolipid protein (PLP) were transfected with an antigen-inducible transgene for platelet-derived growth factor-A (PDGF), a growth factor important in regulating the development of oligodendrocytes. Isolated antigen-specific T cell clones expressed the PDGF transgene when stimulated with PLP 139-151 peptide and produced biologically active PDGF capable of inducing proliferation of oligodendrocyte progenitor cells. Furthermore, upon adoptive transfer, the PDGF transfected T cells migrated to the CNS and ameliorated ongoing disease. Our data indicate that autoreactive memory Th2 cells can be genetically modified so that upon engagement with self antigen they produce regenerative growth factors capable of mediating tissue repair during autoimmune disease. Copyright 1999 Academic Press.

Keywords: JOURNAL ARTICLE Adoptive Transfer Animal Autoantigens Base Sequence Cell Differentiation Cell Movement DNA Primers/GENETICS DNA, Complementary/GENETICS Encephalomyelitis, Experimental Autoimmune/GENETICS/IMMUNOLOGY/ *THERAPY *Gene Therapy *Genetic Vectors Mice Mice, Transgenic Myelin Proteolipid Protein/IMMUNOLOGY Oligodendroglia/PATHOLOGY/PHYSIOLOGY Platelet-Derived Growth Factor/*GENETICS RNA, Messenger/GENETICS Stem Cells/PATHOLOGY/PHYSIOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th2 Cells/*IMMUNOLOGY/PHYSIOLOGY Transfection

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