Influence of nucleotide polymorphisms in the CCR2 gene and the CCR5 promoter on the expression of cell surface CCR5 and CXCR4. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Influence of nucleotide polymorphisms in the CCR2 gene and the CCR5 promoter on the expression of cell surface CCR5 and CXCR4.

Int Immunol. 2000 Sep;12(9):1311-8. Unique Identifier : AIDSLINE MED/20425422
Shieh B; Liau YE; Hsieh PS; Yan YP; Wang ST; Li C; Department of Biochemistry, Chung Shan Medical and Dental; College, No. 110, Section 1, Chien Kuo North Road, Taichung 402,; Taiwan, ROC.

Abstract: Polymorphisms in the CCR2 gene (CCR2-64I) and the CCR5 promoter (pCCR5-59029G) have been correlated with slower HIV-1 disease progression. How these polymorphisms influence the rate of AIDS progression has remained unclear. We have therefore investigated whether these nucleotide polymorphisms will reduce the expression levels of surface CCR5 and CXCR4, and thus lead to slower AIDS progression. For this, a cohort of Chinese volunteers in Taiwan was subjected to the determination of CCR2 and pCCR5 genotypes followed by analysis of the surface CCR5 and CXCR4 expression on five cell types derived from peripheral blood mononuclear cells by flow cytometry. Several significant associations were detected between genotypes and expression levels of the proteins. The most important finding was that an increased number of CD4(+) cells expressing CCR5 correlated with pCCR5-59029A homozygosity without the interference of both the CCR2-64 and the CCR5 delta 32 (deleted 32 bp) mutations (P: = 0.0453), which is consistent with the previous data on the association of the genotype to AIDS progression. Since different genetic polymorphisms co-exist in human beings, the rate of AIDS progression as well as the risk of rheumatoid arthritis may be governed by the interplay of the array of nucleotide changes and their affected proteins.

Keywords: JOURNAL ARTICLE Adult Alleles Cohort Studies Comparative Study Flow Cytometry Genotype Human HIV Seronegativity Leukocytes, Mononuclear/*METABOLISM Middle Age Polymorphism (Genetics) Receptors, Chemokine/*GENETICS Receptors, CCR5/ANALYSIS/*GENETICS Receptors, CXCR4/*ANALYSIS Support, Non-U.S. Gov't Taiwan

KWDjournalarticleadultallelescohortstudiescomparativestudyflowcytometrygenotypehumanhivseronegativityleukocytes,mononuclear/KWDmetabolismmiddleagepolymorphism(genetics)receptors,chemokine/KWDgeneticsreceptors,ccr5/analysis/KWDgeneticsreceptors,cxcr4/KWDanalysissupport,non-uKWDsKWDgov'ttaiwan
001230
A00C0939

Copyright © 2000 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Elton John AIDS Foundation, the National Library of Medicine, and donations from users like you. Always watch for outdated information. This article first appeared in 2000. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 2000. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .