Monocyte inflammatory protein-1 alpha facilitates priming of CD8(+) T cell responses to exogenous viral antigen. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Monocyte inflammatory protein-1 alpha facilitates priming of CD8(+) T cell responses to exogenous viral antigen.

Int Immunol. 2000 Sep;12(9):1365-70. Unique Identifier : AIDSLINE MED/20425428
Flesch IE; Stober D; Schirmbeck R; Reimann J; Department of Medical Microbiology and Immunology, University of; Ulm, Helmholtzstrasse 8/1, 89081 Ulm, Germany.

Abstract: Dendritic cells (DC) derived from bone marrow precursors of BALB/c or C57BL/6 mice in low-serum cultures supplemented with granulocyte macrophage colony stimulating factor and Flt(3) ligand were pulsed in vitro with hepatitis B surface antigen (HBsAg) particles. DC processed exogenous HBsAg and presented its MHC class I-binding epitopes to cytotoxic T lymphocytes (CTL). This specific and restricted interaction of DC with CTL stimulated release of IFN-gamma and macrophage inflammatory protein (MIP)-1 alpha from the responding CTL. MIP-1 alpha enhanced the survival of DC in vitro but did not induce proliferation. Furthermore, co-delivery of MIP-1 alpha facilitated CTL priming in vivo to exogenous HBsAg in low responder C57BL/6 (H-2(b)) mice: a single injection of a low dose of HBsAg particles (without further adjuvants) successfully primed K(b)-restricted CTL responses to HBsAg only when the exogenous antigen was co-delivered with 100 ng MIP-1 alpha. These in vitro and in vivo data point to an important role of MIP-1 alpha in the DC-dependent priming of CTL to exogenous viral antigens.

Keywords: JOURNAL ARTICLE Adjuvants, Immunologic Animal Cell Division Cells, Cultured Coculture CD8-Positive T-Lymphocytes/*IMMUNOLOGY Dendritic Cells/CYTOLOGY/DRUG EFFECTS/IMMUNOLOGY Granulocyte-Macrophage Colony-Stimulating Factor/PHARMACOLOGY Hepatitis B Surface Antigens/IMMUNOLOGY Histocompatibility Antigens Class I Interferon Type II/ANALYSIS Macrophage Inflammatory Protein-1/ANALYSIS/*IMMUNOLOGY/ PHARMACOLOGY Mice Mice, Inbred BALB C Mice, Inbred C57BL Support, Non-U.S. Gov't T-Lymphocytes, Cytotoxic/IMMUNOLOGY Vaccination

KWDjournalarticleadjuvants,immunologicanimalcelldivisioncells,culturedcoculturecd8-positivet-lymphocytes/
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A00C0937

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