Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
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Gene Ther. 2000 Aug;7(16):1421-4. Unique Identifier : AIDSLINE MED/20434549
Mautino MR; Keiser N; Morgan RA; Clinical Gene Therapy Branch, National Human Genome Research; Institute, National Institutes of Health, Bethesda, MD; 20892-1851, USA.
Abstract: The development of lentiviral vectors that use Rev-independent mechanisms of nuclear export for their genomic RNA could facilitate the construction of novel anti-HIV vectors. We have improved the titers of Rev-independent lentiviral vectors having the SRV-1 CTE by mutating the major splice donor and acceptor sites present in the vector and by relocalization of the CTE sequences adjacent to the HIV-1 3'LTR. These two modifications have additive beneficial effects on vector titers and packaging efficiency. Packaging these CTE+ vectors expressing marker genes with a Rev-dependent HIV-1 helper vector yields higher titers than are obtained using a Rev-dependent lentiviral vector.
001230
A00C0923
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