Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
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RNA. 2000 Sep;6(9):1325-34. Unique Identifier : AIDSLINE MED/20452238
Ghosh S; Garcia-Blanco MA; Department of Genetics, Duke University Medical Center, Durham,; North Carolina 27710, USA.
Abstract: Compelling in vivo studies suggest a tight functional linkage between RNA polymerase II transcription and premessenger RNA splicing. At present, the specific interactions involved in this coupling are poorly understood and deserve investigation. To this end, we developed an in vitro system that permits study of coupled transcription and splicing. Transcripts generated by RNA polymerase II were accurately and efficiently spliced under reaction conditions that permitted both transcription and splicing to occur simultaneously. The splicing of RNA-polymerase-II-driven transcripts was accelerated relative to that of the same transcripts driven by T7 RNA polymerase. Moreover, the product of exon ligation was found associated with the DNA template in reactions driven by RNA polymerase II. These two findings indicate that transcription and splicing were coupled in the in vitro system driven by RNA polymerase II, and suggest that this system will be useful for the biochemical study of this coupling.
001230
A00C0919
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