Spleen necrosis virus-derived C-type retroviral vectors for gene transfer to quiescent cells. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Spleen necrosis virus-derived C-type retroviral vectors for gene transfer to quiescent cells.

Nat Biotechnol. 2000 Jun;18(6):623-9. Unique Identifier : AIDSLINE MED/20296926
Parveen Z; Krupetsky A; Engelstadter M; Cichutek K; Pomerantz RJ; Dornburg R; The Dorrance H. Hamilton Laboratories, Thomas Jefferson; University, Division of Infectious Diseases, Center for Human; Virology, 1020 Locust Street, Suite 329, Philadelphia, PA 19107,; USA.

Abstract: Gene therapy applications of retroviral vectors derived from C-type retroviruses have been limited to introducing genes into dividing target cells. Here, we report genetically engineered C-type retroviral vectors derived from spleen necrosis virus (SNV), which are capable of infecting nondividing cells. This has been achieved by introducing a nuclear localization signal (NLS) sequence into the matrix protein (MA) of SNV by site-directed mutagenesis. This increased the efficiency of infecting nondividing cells and was sufficient to endow the virus with the capability to efficiently infect growth-arrested human T lymphocytes and quiescent primary monocyte-derived macrophages. We demonstrate that this vector actively penetrates the nucleus of a target cell, and has potential use as a gene therapy vector to transfer genes into nondividing cells.

Keywords: JOURNAL ARTICLE Amino Acid Sequence Animal Blotting, Southern Cell Division Cell Line Cell Nucleus/VIROLOGY Dogs Extracellular Matrix Proteins/GENETICS Flow Cytometry Gene Products, gag/GENETICS *Gene Transfer Genetic Vectors/*METABOLISM Hela Cells Human Jurkat Cells Macrophages/VIROLOGY Molecular Sequence Data Monocytes/VIROLOGY Mutagenesis, Site-Directed Necrosis Nuclear Localization Signal/GENETICS Plasmids Retroviruses Type C, Mammalian/*METABOLISM Spleen/VIROLOGY Support, U.S. Gov't, P.H.S. T-Lymphocytes/VIROLOGY Transfection Tumor Cells, Cultured

KWDjournalarticleaminoacidsequenceanimalblotting,southerncelldivisioncelllinecellnucleus/virologydogsextracellularmatrixproteins/geneticsflowcytometrygeneproducts,gag/geneticsKWDgenetransfergeneticvectors/KWDmetabolismhelacellshumanjurkatcellsmacrophages/virologymolecularsequencedatamonocytes/virologymutagenesis,site-directednecrosisnuclearlocalizationsignal/geneticsplasmidsretrovirusestypec,mammalian/KWDmetabolismspleen/virologysupport,uKWDsKWDgov't,pKWDhKWDsKWDt-lymphocytes/virologytransfectiontumorcells,cultured
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