Importance of the CD3 marker for evaluating changes in rhesus macaque CD4/CD8 T-cell ratios.

Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

Importance of the CD3 marker for evaluating changes in rhesus macaque CD4/CD8 T-cell ratios.

Cytometry. 2000 May 1;40(1):69-75. Unique Identifier : AIDSLINE MED/20218908
Dykhuizen M; Ceman J; Mitchen J; Zayas M; MacDougall A; Helgeland J; Rakasz E; Pauza CD; Wisconsin Regional Primate Research Center, Madison, WI 53706,; USA.

Abstract: BACKGROUND: Until recently, there were no CD3 antibodies that crossreacted with rhesus macaque T cells. Consequently, studies relying on CD8 counts or CD4/CD8 ratios enumerated this subpopulation on the basis of CD8+ or CD8bright+ staining. We used a rhesus-specific, anti-CD3 antibody to better define the CD8+ T-cell population, and to show the effects of better measurements on CD4/CD8 ratios and changes in T cells as macaques age. METHODS: We used three-color flow cytometry to measure CD4 and CD8 populations with and without CD3 costaining. Venous blood samples were obtained from 52 colony-bred macaques between 2 months and 9 years of age. RESULTS: The CD8+ T cells defined by CD3 and CD8 double staining were approximately 60% of all cells that were stained by CD8 alone. Improved detection of this lymphocyte subset showed that CD4/CD8 ratios were close to the range of 1.5-2.0. Declining CD4/CD8 ratios during aging are predominantly due to decreasing CD4+ T-cell counts. CONCLUSIONS: Better quantitation of the CD8+ T-cell population showed that the CD4/CD8 ratio was not inverted as had been reported, but is actually very similar to the values observed in human beings. Although the two species differ in the pattern of CD8 expression, the general immune system characteristics are very similar. Copyright 2000 Wiley-Liss, Inc.

Keywords: JOURNAL ARTICLE Age Factors Animal Antigens, CD3/*ANALYSIS/*IMMUNOLOGY Antigens, CD4/ANALYSIS/IMMUNOLOGY Antigens, CD8/ANALYSIS/IMMUNOLOGY Cross Reactions CD4-CD8 Ratio/*METHODS CD4-Positive T-Lymphocytes/CHEMISTRY/IMMUNOLOGY CD8-Positive T-Lymphocytes/CHEMISTRY/IMMUNOLOGY Female Flow Cytometry/*METHODS Macaca mulatta Male Support, U.S. Gov't, P.H.S.

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A0080978

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