Characterization of the ovalbumin-specific TCR transgenic line OT-I: MHC elements for positive and negative selection.

Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

Characterization of the ovalbumin-specific TCR transgenic line OT-I: MHC elements for positive and negative selection.

Immunol Cell Biol. 2000 Apr;78(2):110-7. Unique Identifier : AIDSLINE MED/20225765
Clarke SR; Barnden M; Kurts C; Carbone FR; Miller JF; Heath WR; Immunology Division, The Walter and Eliza Hall Institute of; Medical Research, Melbourne, Victoria, Australia.

Abstract: The present report provides the first extensive characterization of the OT-I TCR transgenic line, which produces MHC class I-restricted, ovalbumin-specific, CD8+ T cells (OT-I cells). These cells are shown to be positively selected in vivo in H-2b C57BL/6 mice and in bm5 mice, which express the Kbm5 mutant molecule. In contrast, OT-I cells were not selected by mutant Kb molecules in bm1, bm3, bm8, bm10, bm11 or bm23 mice. Interestingly, however, when positive selection was examined in vitro in foetal thymic organ culture (FTOC), bm1 and bm8 were still poorly selective, but the bm3 haplotype now selected as efficiently as B6. The ability to select in vitro correlated with the capacity to present the ovalbumin (OVA) peptide to OT-I cells, as measured by induction of an OVA-specific proliferative response. These results suggest that a lower affinity TCR:MHC interaction may be necessary for positive selection in FTOC compared with selection in situ.

Keywords: JOURNAL ARTICLE Animal Antigens, CD4/ANALYSIS Antigens, CD8/ANALYSIS Cells, Cultured Comparative Study Cytotoxicity, Immunologic CD8-Positive T-Lymphocytes/IMMUNOLOGY/METABOLISM Flow Cytometry Haplotypes Histocompatibility Antigens Class I/*METABOLISM Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Transgenic/GENETICS/*IMMUNOLOGY Mutation Ovalbumin/CHEMISTRY/*METABOLISM Receptors, Antigen, T-Cell/GENETICS/*METABOLISM Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.

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A0080970

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