[Use of polymerase chain reaction in medical diagnosis] NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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[Use of polymerase chain reaction in medical diagnosis]

Przegl Lek. 1999;56(11):723-34. Unique Identifier : AIDSLINE MED/20260380
Swiat A; Stepien E; Andres J; Dziatkowiak A; Katedry Anestezjologii i Intensywnej Terapii, Collegium Medicum,; Uniwersytetu Jagiellonskiego w Krakowie.

Abstract: Principles of the polymerase chain reaction (PCR) and its modifications are discussed: reverse transcription-PCR (RT-PCR), nested PCR, restriction fragment length polymorphism-PCR (RFLP-PCR). Different methods of the PCR products detection used in routine diagnostics and research are presented: electrophoresis and chromatography techniques, immunoenzymatic methods, hybridisation and sequencing. Applications of PCR reaction in diagnostics of infectious diseases (viral and bacterial), genetic diseases and genotyping in transplantation are discussed and examples of commercial tests based on PCR reaction and another molecular biology methods used in fast, routine diagnostics are listed.

Keywords: JOURNAL ARTICLE REVIEW REVIEW, TUTORIAL medycznej. Acquired Immunodeficiency Syndrome/DIAGNOSIS Bacterial Infections/*DIAGNOSIS Chromatography/METHODS Electrophoresis/METHODS English Abstract Genetics, Biochemical/METHODS Genotype Hereditary Diseases/*DIAGNOSIS Human Polymerase Chain Reaction/*METHODS Tuberculosis/DIAGNOSIS Virus Diseases/*DIAGNOSIS

KWDjournalarticlereviewreview,tutorialmedycznejKWDacquiredimmunodeficiencysyndrome/diagnosisbacterialinfections/KWDdiagnosischromatography/methodselectrophoresis/methodsenglishabstractgenetics,biochemical/methodsgenotypehereditarydiseases/KWDdiagnosishumanpolymerasechainreaction/KWDmethodstuberculosis/diagnosisvirusdiseases/KWDdiagnosis
000830
A0080921

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