Changes in human immunodeficiency virus type 1 virus load during mobilization and harvesting of hemopoietic progenitor cells. Adult AIDS Clinical Trials Group 285 Study Team.

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Changes in human immunodeficiency virus type 1 virus load during mobilization and harvesting of hemopoietic progenitor cells. Adult AIDS Clinical Trials Group 285 Study Team.

Blood. 2000 Jan 1;95(1):48-55. Unique Identifier : AIDSLINE MED/20076238
Campbell TB; Sevin A; Coombs RW; Peterson GC; Rosandich M; Kuritzkes DR; Mladenovic J; Landay A; Wong R; Ambruso D; Miles S; Pomerantz RJ; Schooley RT; University of Colorado Health Sciences Center and Bonfils Blood; Center, Denver, CO 80262, USA. thomas.campbell@uchsc.edu

Abstract: Genetic modification of hemopoietic progenitor cells ex vivo, followed by the infusion of the genetically modified cells into the human immunodeficiency virus-1 (HIV-1) infected donor, has been proposed as a treatment for HIV-1 infection. The current study was undertaken to evaluate the effect of hemopoietic stem cell mobilization and harvesting on HIV-1 replication in persons with HIV-1 infection. Eighteen HIV-1-infected persons received recombinant granulocyte colony-stimulating factor (G-CSF; Filgrastim) 10 microg/kg per day, for 7 days. On days 4 and 5, peripheral blood mononuclear cells were harvested by leukapheresis. The CD4+ lymphocyte count at entry was >500/microL for 6 subjects, 200 to 500/microL for 6 subjects, and <200/microL for 6 subjects. For 9 of 18 subjects, plasma HIV-1 RNA levels increased 4- to 100-fold (>0.6 log(10)) above baseline between days 4 and 7 and returned to baseline by day 27. Significant increases of plasma HIV-1 RNA levels occurred in 5 subjects despite 3-drug antiretroviral therapy. Changes in CD4+ and CD34+ cells during mobilization and harvesting were similar in all subjects whether they had or did not have increased plasma HIV-1 RNA levels. Thus, mobilization and harvesting of bone marrow progenitor cells from persons infected with HIV-1 induced a transient increase in viral replication in some patients but was not associated with adverse effects. (Blood. 2000;95: 48-55)

Keywords: JOURNAL ARTICLE Adult Cohort Studies CD4 Lymphocyte Count DNA, Viral/BLOOD Filgrastim/*THERAPEUTIC USE *Hematopoietic Stem Cell Mobilization Hematopoietic Stem Cells/*PATHOLOGY Human HIV Infections/*BLOOD/IMMUNOLOGY/THERAPY HIV-1/*ISOLATION & PURIF *Leukapheresis Lymphocytes/*VIROLOGY Male Middle Age Patient Selection RNA, Viral/BLOOD Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. *Viral Load

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