Pertussis toxin treatment in vivo reduces surface expression of the adhesion integrin leukocyte function antigen-1 (LFA-1). NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Pertussis toxin treatment in vivo reduces surface expression of the adhesion integrin leukocyte function antigen-1 (LFA-1).

Cell Adhes Commun. 1999;7(3):183-93. Unique Identifier : AIDSLINE MED/20090329
Schenkel AR; Pauza CD; Department of Pathology and Laboratory Medicine and Wisconsin; Regional Primate Research Center, University of; Wisconsin-Madison, 53706, USA.

Abstract: Pertussis toxin treatment in macaques inhibits lymphocyte extravasation from the blood and leads to transient lymphocytosis and leukocytosis. We examined lymphocyte adhesion molecules known to be involved in the extravasation process to find possible mechanisms for the effects of pertussis toxin treatment. The two subunits of LFA-1, CD11a and CD18, showed decreased surface expression on lymphocytes from pertussis toxin treated animals compared to untreated animals. The adhesion molecule CD44, and the alpha subunit of the integrin VLA-4 (CD49d) were not decreased by pertussis toxin treatment. Lower surface expression of CD11a and CD18 was observed on all lymphocyte subsets and was correlated inversely with the extent of lymphocytosis. The magnitude of lymphocytosis after pertussis toxin treatment was higher in SIV-infected macaques than in uninfected animals. However, changes in LFA-1 levels were similar in both groups. These data show that LFA-1 surface levels are affected by pertussis toxin in vivo and this change may account in part, for the ability of pertussis toxin to induce lymphocytosis.

Keywords: JOURNAL ARTICLE Animal Antigens, CD18/BIOSYNTHESIS Antigens, CD20/METABOLISM Cell Count/DRUG EFFECTS CD4-Positive T-Lymphocytes/METABOLISM CD8-Positive T-Lymphocytes/METABOLISM Flow Cytometry Gene Expression/DRUG EFFECTS Lymphocyte Function-Associated Antigen-1/*BIOSYNTHESIS/GENETICS Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY Lymphocytosis Macaca mulatta Pertussis Toxins/*PHARMACOLOGY Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGY Support, U.S. Gov't, P.H.S. Time Factors


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