High-performance liquid chromatographic method for the simultaneous determination of HIV-1 protease inhibitors indinavir, saquinavir and ritonavir in plasma of patients during highly active antiretroviral therapy. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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High-performance liquid chromatographic method for the simultaneous determination of HIV-1 protease inhibitors indinavir, saquinavir and ritonavir in plasma of patients during highly active antiretroviral therapy.

J Chromatogr B Biomed Sci Appl. 1999 Nov 26;735(1):41-50. Unique Identifier : AIDSLINE MED/20094585
Langmann P; Klinker H; Schirmer D; Zilly M; Bienert A; Richter E; Department of Internal Medicine, University of Wurzburg,; Germany.

Abstract: A new high-performance liquid chromatographic method for the simultaneous determination of indinavir, saquinavir and ritonavir in human plasma is described. Quantitative recovery following liquid-liquid extraction with diethyl ether from 500 microl of human plasma was achieved. Subsequently, the assay was performed with a linear gradient starting at 67 mM potassium dihydrogenphosphate-acetonitrile (65:35 to 40:60, v/v) as a mobile phase, a Phenomenex C18 column and UV detection at 240 and 258 nm, respectively. Linear standard curves were obtained for concentrations ranging from 75 to 20,000 ng/ml for indinavir, from 10 to 6000 ng/ml for saquinavir, and from 45 to 30,000 ng/ml for ritonavir. The calculated intra- and inter-day coefficients of variation were below 6%.

Keywords: JOURNAL ARTICLE Chromatography, High Pressure Liquid/*METHODS Drug Stability Drug Therapy, Combination Human HIV Infections/BLOOD/*DRUG THERAPY HIV Protease Inhibitors/*BLOOD Indinavir/*BLOOD Kinetics Ritonavir/*BLOOD Saquinavir/*BLOOD Sensitivity and Specificity

KWDjournalarticlechromatography,highpressureliquid/KWDmethodsdrugstabilitydrugtherapy,combinationhumanhivinfections/blood/KWDdrugtherapyhivproteaseinhibitors/KWDbloodindinavir/KWDbloodkineticsritonavir/KWDbloodsaquinavir/KWDbloodsensitivityandspecificity
000430
A0040878

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