Epitope mapping of rat neutralizing monoclonal antibody against human immunodeficiency virus type-1 by a phage peptide library: comparison with ELISA using synthetic peptides.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Epitope mapping of rat neutralizing monoclonal antibody against human immunodeficiency virus type-1 by a phage peptide library: comparison with ELISA using synthetic peptides.

Viral Immunol. 1999;12(1):57-66. Unique Identifier : AIDSLINE MED/99264038
Ichiyama K; Ishikawa D; Tanaka Y; Kashiwa T; Koyanagi Y; Handa S; Yamashita A; Fukushi M; Yamamoto N; Taki T; Department of Microbiology, Tokyo Medical and Dental University; School of Medicine, Japan.

Abstract: We generated a rat monoclonal antibody (mAb W#10) with the ability to neutralize human immunodeficiency virus type 1IIIB (HIV-1IIIB) infection. The epitope recognized by mAb W#10 was defined as R-I-Q-R-G-P-G by enzyme-linked immunosorbent assay (ELISA) with the use of synthetic peptides. The filamentous phage clones displaying random 15-amino-acid peptides on the amino terminus of the pIII coat protein reacting with mAb W#10 were identified with affinity and immunological selection procedures. Thirteen out of 16 selected phage clones contained the G-X-G-R-X-F sequence in the coat protein region representing significant homology to a part of conserved G-P-G-R-A-F sequence in the V3 loop of various HIV-1 strains. In addition, the phage clones included the G-X-G sequence in the sequence detected by synthetic peptides as the recognition site. The selected phage clones were stained by mAb W#10 specifically and were able to compete with mAb binding to cells expressing viral antigens.

Keywords: JOURNAL ARTICLE Amino Acid Sequence Animal Antibodies, Monoclonal/*CHEMISTRY Binding Sites, Antibody Cells, Cultured Coliphages/GENETICS/IMMUNOLOGY Comparative Study Enzyme-Linked Immunosorbent Assay/METHODS Epitope Mapping/*METHODS Human HIV-1/*IMMUNOLOGY Kidney Molecular Sequence Data Neutralization Tests *Peptide Library Peptide Mapping Peptides/ANALYSIS/*CHEMICAL SYNTHESIS/*IMMUNOLOGY Rats Support, Non-U.S. Gov't
990930
A9991373

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