Quantification of RNA in HIV type 1 subtypes D and G by NucliSens and Amplicor assays in Abidjan, Ivory Coast. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Quantification of RNA in HIV type 1 subtypes D and G by NucliSens and Amplicor assays in Abidjan, Ivory Coast.

AIDS Res Hum Retroviruses. 1999 Apr 10;15(6):495-8. Unique Identifier : AIDSLINE MED/99236715
Nkengasong JN; Bile C; Kalou M; Maurice C; Boateng E; Sassan-Morokro M; Rayfield M; Coulibaly D; Greenberg AE; Wiktor SZ; Projet RETRO-CI, CHU Treichville, Abidjan, Ivory Coast.; jcn5@cdc.gov

Abstract: We have compared the performance of the NucliSens and the standard and modified HIV Monitor assays to quantify HIV-1 RNA plasma viral load in 12 tuberculosis patients infected with HIV-1 env subtype D (n = 3) and env subtype G (n = 9) in Ivory Coast. RNA was quantified in all nine subtype G specimens by the modified Amplicor HIV Monitor (mean, 4.6 log10 copies/ml; range, 3.1-6.3 log10/ml), in seven specimens by NucliSens (mean, 4.4 log10 copies/ml; range, 2.7-5.5 log10 copies/ml), and in 6 specimens by the standard Amplicor HIV Monitor assay (mean, 4.2 log10 copies/ml; range, 3.5-5.0 log10 copies/ml). All three subtype D samples were amplified by both the modified Amplicor HIV Monitor (mean, 4.5 log10 copies/ml; range, 3.8-5.1 log10 copies/ml) and NucliSens (mean, 3.8 log10 copies/ml; range, 2.8-5.0 log10 copies/ml); two samples were quantified by the standard Amplicor HIV Monitor assay (mean, 3.0 log10 copies/ml; range, 2.4-3.6 log10 copies/ml). Our preliminary results suggest that the modified Amplicor HIV Monitor can accurately quantify HIV-1 RNA viral load in persons infected with subtype D and G strains.
Keywords: JOURNAL ARTICLE AIDS-Related Opportunistic Infections/BLOOD/*VIROLOGY Cote d'Ivoire Human *HIV-1/GENETICS *Reagent Kits, Diagnostic RNA, Viral/*BLOOD Support, U.S. Gov't, P.H.S. Tuberculosis/BLOOD/*VIROLOGY *Viral LoadKWDjournalarticleaids-relatedopportunisticinfections/blood/KWDvirologycoted'ivoirehumanKWDhiv-1/geneticsKWDreagentkits,diagnosticrna,viral/KWDbloodsupport,uKWDsKWDgov't,pKWDhKWDsKWDtuberculosis/blood/KWDvirologyKWDviralload
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