Comparison of enzymatic activities of the HIV-1 and HFV integrases to their U5 LTR substrates. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Comparison of enzymatic activities of the HIV-1 and HFV integrases to their U5 LTR substrates.

Biochem Mol Biol Int. 1999 Apr;47(4):621-9. Unique Identifier : AIDSLINE MED/99252972
Oh YT; Shin CG; Department of Biotechnology, Chung-Ang University, Kyungki,; Korea.

Abstract: The human immunodeficiency virus type 1 (HIV-1) and human foamy virus (HFV) integrase proteins were overexpressed in Escherichia coli, and purified to a near homogeneity by one- or two-step purification scheme. The endonucleolytic, integration, and disintegration activities for the HIV-1 and HFV integrases were characterized in vitro. The endonucleolytic activities for the HIV-1 and HFV integrases were found only on their own substrates, respectively, indicating that the cognate U5 LTR sequences in the substrates is critical for specific cleavage. However, the integration and disintegration activities showed less specificity on the substrate usage. Our results suggest that the disintegration activity have more preference for substrates based on Y-shaped structure rather than on viral donor DNA sequence.
Keywords: JOURNAL ARTICLE Comparative Study Gene Expression Human HIV Integrase/GENETICS/ISOLATION & PURIF/*METABOLISM *HIV Long Terminal Repeat HIV-1/*ENZYMOLOGY Integrase/GENETICS/ISOLATION & PURIF/*METABOLISM Recombinant Fusion Proteins/GENETICS/ISOLATION & PURIF/METABOLISM Ribonucleoproteins, Small, U5/*GENETICS Spumavirus/*ENZYMOLOGY Substrate Specificity Support, Non-U.S. Gov't *Terminal Repeat SequencesKWDjournalarticlecomparativestudygeneexpressionhumanhivintegrase/genetics/isolation&purif/KWDmetabolismKWDhivlongterminalrepeathiv-1/KWDenzymologyintegrase/genetics/isolation&purif/KWDmetabolismrecombinantfusionproteins/genetics/isolation&purif/metabolismribonucleoproteins,small,u5/KWDgeneticsspumavirus/KWDenzymologysubstratespecificitysupport,non-uKWDsKWDgov'tKWDterminalrepeatsequences
991030
A99A0940

Copyright © 1999 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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