Immunization of RANTES expression plasmid with a DNA vaccine enhances HIV-1-specific immunity. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Immunization of RANTES expression plasmid with a DNA vaccine enhances HIV-1-specific immunity.

Clin Immunol. 1999 Jul;92(1):90-6. Unique Identifier : AIDSLINE MED/99343954
Xin KQ; Lu Y; Hamajima K; Fukushima J; Yang J; Inamura K; Okuda K; Department of Bacteriology, Yokohama City University School of; Medicine, Yokohama, 236, Japan.

Abstract: Cytokines play important roles in regulating immune response. This study evaluated the adjuvant effect of an expression plasmid encoding RANTES (regulated on activation normal T-cell expressed and secreted) chemokine on the immunity induced by a DNA vaccine. This vaccine consists of expression plasmids encoding the env and rev genes of human immunodeficiency virus type 1 (HIV-1). DNA vaccination with RANTES plasmid induced significantly higher titers of serum HIV-1-specific IgG and IgG2a antibodies than DNA vaccination alone on both intramuscular and intranasal immunization. This combination also increased HIV-1-specific cytotoxic T lymphocyte activity and delayed-type hypersensitivity. Intranasal immunization induced a higher titer of fecal secretory IgA antibody than intramuscular immunization. These results demonstrate that coadministration of RANTES plasmid dominantly induced HIV-1-specific cell-mediated immunity. Copyright 1999 Academic Press.
Keywords: JOURNAL ARTICLE Adjuvants, Immunologic Animal Antibodies, Viral/IMMUNOLOGY Antibody Formation Antibody Specificity Female Histiocytes/CHEMISTRY/CYTOLOGY Hypersensitivity, Delayed/IMMUNOLOGY/PATHOLOGY/VIROLOGY HIV-1/*IMMUNOLOGY Immunity, Cellular/IMMUNOLOGY Lymphocytes/CHEMISTRY/CYTOLOGY Mice Mice, Inbred BALB C Muscle, Skeletal/CYTOLOGY RANTES/*IMMUNOLOGY Support, Non-U.S. Gov't Vaccines, DNA/*CHEMISTRY/*IMMUNOLOGYKWDjournalarticleadjuvants,immunologicanimalantibodies,viral/immunologyantibodyformationantibodyspecificityfemalehistiocytes/chemistry/cytologyhypersensitivity,delayed/immunology/pathology/virologyhiv-1/KWDimmunologyimmunity,cellular/immunologylymphocytes/chemistry/cytologymicemice,inbredbalbcmuscle,skeletal/cytologyrantes/KWDimmunologysupport,non-uKWDsKWDgov'tvaccines,dna/KWDchemistry/KWDimmunology
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