New substrates of DNA polymerases.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

New substrates of DNA polymerases.

FEBS Lett. 1999 Jun 18;453(1-2):6-10. Unique Identifier : AIDSLINE MED/99330158
Victorova L; Sosunov V; Skoblov A; Shipytsin A; Krayevsky A; Engelhardt Institute of Molecular Biology, Russian Academy of; Sciences, Moscow.

Abstract: Bis-(2'-deoxynucleoside) 5',5'-tetraphosphates and bis-(2'-deoxynucleoside) 5',5'-triphosphates were shown to be a new type of substrate for several DNA polymerases of human, bacterial and viral origin. Their substrate properties depend both on their structure and on the nature of the enzyme. They are incorporated by both termini in correspondence with the template nucleotide program in the active center. The results obtained support the mechanism of their direct incorporation rather than prior hydrolysis to dNTP. The highest activity of these compounds was observed for HIV reverse transcriptase. The probable biological significance of the reaction is discussed.

Keywords: JOURNAL ARTICLE Bacteria/ENZYMOLOGY Comparative Study Dinucleoside Phosphates/*CHEMISTRY/*METABOLISM DNA Polymerase I/METABOLISM DNA-Directed DNA Polymerase/*METABOLISM Human RNA-Directed DNA Polymerase/METABOLISM Substrate Specificity Support, Non-U.S. Gov't Taq Polymerase/METABOLISM Viruses/ENZYMOLOGY
991030
A99A0889

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