Isolation and characterization of ARA160 as the first androgen receptor N-terminal-associated coactivator in human prostate cells.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Isolation and characterization of ARA160 as the first androgen receptor N-terminal-associated coactivator in human prostate cells.

J Biol Chem. 1999 Aug 6;274(32):22373-9. Unique Identifier : AIDSLINE MED/99357764
Hsiao PW; Chang C; Departments of Pathology, Urology, and Radiation Oncology, George; Whipple Laboratory for Cancer Research, University of Rochester,; Rochester, New York 14642, USA.

Abstract: The androgen receptor (AR) is a member of the steroid receptor superfamily that may require coactivators for proper or maximal transactivation. Using a purified AR N-terminal peptide as a probe to screen the human testis expression library, we identified an androgen-enhanced AR N-terminal-associated protein ARA160, which consists of 1,093 amino acids with an apparent molecular mass of 160 kDa. Sequence comparison in GenBank(TM) reveals that ARA160 shares an identical sequence with a HIV-1 TATA element modulatory factor, TMF. The far-Western blotting and co-immunoprecipitation assays demonstrate that the AR can interact directly with ARA160/TMF. Affinity gel pull-down and mammalian two-hybrid assays further suggest androgen can enhance significantly the interaction between AR and ARA160. Transient transfection assays demonstrated that ARA160 might function as a coactivator for AR-mediated transactivation in human prostate cancer PC-3 cells. Our data further suggest that this AR N-terminal coactivator can function cooperatively with AR C-terminal coactivator, ARA70, in PC-3 cells. Together, our data demonstrate that ARA160 might represent the first identified androgen-enhanced N-terminal coactivator for the AR.

Keywords: JOURNAL ARTICLE Amino Acid Sequence Androgens/*METABOLISM Cloning, Molecular DNA-Binding Proteins/GENETICS/ISOLATION & PURIF/*METABOLISM Human Ligands Male Mammary Tumor Viruses, Mouse/GENETICS Molecular Sequence Data Promoter Regions (Genetics) Prostate/CHEMISTRY/*METABOLISM Prostate-Specific Antigen/GENETICS Protein Binding Receptors, Androgen/*METABOLISM Receptors, Estrogen/METABOLISM Receptors, Glucocorticoid/METABOLISM Response Elements Sequence Analysis, DNA Support, U.S. Gov't, P.H.S. Terminal Repeat Sequences Trans-Activation (Genetics) Trans-Activators/METABOLISM Transcription Factors/GENETICS/ISOLATION & PURIF/*METABOLISM
991130
A99B0961

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