Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response.

Immunity. 1999 Jan;10(1):127-36. Unique Identifier : AIDSLINE GENBANK/AF110460
Novick D; Kim SH; Fantuzzi G; Reznikov LL; Dinarello CA; Rubinstein M; Department of Molecular Genetics, Weizmann Institute of Science,; Rehovot, Israel.

Abstract: An interleukin-18 binding protein (IL-18BP) was purified from urine by chromatography on IL-18 beads, sequenced, cloned, and expressed in COS7 cells. IL-18BP abolished IL-18 induction of interferon-gamma (IFNgamma), IL-8, and activation of NF-kappaB in vitro. Administration of IL-18BP to mice abrogated circulating IFNgamma following LPS. Thus, IL-18BP functions as an inhibitor of the early Th1 cytokine response. IL-18BP is constitutively expressed in the spleen, belongs to the immunoglobulin superfamily, and has limited homology to the IL-1 type II receptor. Its gene was localized on human chromosome 11q13, and no exon coding for a transmembrane domain was found in an 8.3 kb genomic sequence. Several Poxviruses encode putative proteins highly homologous to IL-18BP, suggesting that viral products may attenuate IL-18 and interfere with the cytotoxic T cell response.

Keywords: JOURNAL ARTICLE Amino Acid Sequence Animal Base Sequence Carrier Proteins/GENETICS/ISOLATION & PURIF/*PHYSIOLOGY/URINE Cloning, Molecular Cytokines/*BIOSYNTHESIS COS Cells DNA, Complementary/ISOLATION & PURIF Exons Human Injections, Intraperitoneal Interferon Inducers/ANTAGONISTS & INHIB Interferon Type II/ANTAGONISTS & INHIB/BIOSYNTHESIS Interleukin-18/ANTAGONISTS & INHIB/*METABOLISM/PHYSIOLOGY Introns Lipopolysaccharides/ANTAGONISTS & INHIB/PHARMACOLOGY Mice Molecular Sequence Data Poxviridae/GENETICS Recombinant Proteins/ADMINISTRATION & DOSAGE Sequence Homology, Amino Acid Spleen/METABOLISM Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th1 Cells/*METABOLISM Viral Proteins/GENETICS
990530
A9950931

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