Immunological analysis of rat renal transplant recipients exhibiting long-term survival following treatment with 15-deoxyspergualin in the early postoperative phase.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Immunological analysis of rat renal transplant recipients exhibiting long-term survival following treatment with 15-deoxyspergualin in the early postoperative phase.

Int J Urol. 1998 Sep;5(5):476-81. Unique Identifier : AIDSLINE MED/98452698
Takeuchi I; Chikaraishi T; Seki T; Kanagawa K; Koyanagi T; Department of Urology, Hokkaido University School of Medicine,; Sapporo, Japan.

Abstract: BACKGROUND: We previously reported that short-term administration of 15-deoxyspergualin (DSG), 5 mg/kg/day on postoperative days 4 through 7, prolonged survival of rat renal allografts indefinitely. We now report the immunologic environment of DSG-treated recipients in the early postoperative phase. METHODS: TO (RT1u) rat kidneys were transplanted into WKAH (RT1k) rats. Peripheral blood lymphocytes (PBL), splenocytes (SPC) and graft infiltrating lymphocytes (GIL) were harvested from rejecting (untreated) recipients on day 7 (group AR) and from DSG-treated recipients on days 7 (Group DSG7) and 14 (group DSG14). Flow cytometric analysis was done to determine characteristics of these cells. Mixed lymphocyte culture reactions (MLRs) were also studied to examine suppressive activities of sera, SPC, and GIL of each group by adding them to TO/WKAH MLRs. RESULTS: In all groups, the proportions of CD8- and interleukin 2 receptor (IL-2R)-positive cells were higher for GIL than for either PBL or SPC. The CD4/CD8 ratio was lowest in GIL. Comparing groups DSG7 and DSG14, significant decreases in the proportion of CD8- and IL-2R-positive cells were found only in GIL. Sera of all groups nonspecifically suppressed MLRs, independent of DSG-administration. GIL of all groups also nonspecifically suppressed MLRs, while these suppressive activities were not observed with SPC. Suppressive activities of GIL remained unchanged in the first 2 postoperative weeks. CONCLUSIONS: Differences in the immunologic environment were reflected primarily in GIL. DSG seemed to decrease CD8- and IL-2R-positive cells in allografts. In the presence of DSG, this model may feature a predominance of nonspecific suppressor T cells over cytotoxic T cells during the first 2 postoperative weeks.

Keywords: JOURNAL ARTICLE Animal Cells, Cultured Comparative Study CD4-CD8 Ratio CD4-Positive T-Lymphocytes/IMMUNOLOGY CD8-Positive T-Lymphocytes/IMMUNOLOGY Follow-Up Studies Graft Rejection/BLOOD/IMMUNOLOGY/*PREVENTION & CONTROL Graft Survival/*DRUG EFFECTS/IMMUNOLOGY Guanidines/*THERAPEUTIC USE Immunosuppressive Agents/*THERAPEUTIC USE Kidney Transplantation/*IMMUNOLOGY/PATHOLOGY Male Postoperative Period Rats Rats, Inbred Strains Receptors, Interleukin-2/BLOOD Spleen/IMMUNOLOGY/PATHOLOGY
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A9931104

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