Cloning and sequencing of yajC and secD homologs of Brucella abortus and demonstration of immune responses to YajC in mice vaccinated with B. abortus RB51.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Cloning and sequencing of yajC and secD homologs of Brucella abortus and demonstration of immune responses to YajC in mice vaccinated with B. abortus RB51.

Infect Immun. 1998 Dec;66(12):5684-91. Unique Identifier : AIDSLINE GENBANK/AF085217
Vemulapalli R; Duncan AJ; Boyle SM; Sriranganathan N; Toth TE; Schurig GG; Center for Molecular Medicine and Infectious Diseases, Department; of Biomedical Sciences and Pathobiology, VA-MD Regional College; of Veterinary Medicine, Virginia Polytechnic Institute and State; University, Blacksburg, Virginia 24061-0342, USA.

Abstract: To identify Brucella antigens that are potentially involved in stimulating a protective cell-mediated immune response, a gene library of Brucella abortus 2308 was screened for the expression of antigens reacting with immunoglobulin G2a antibodies from BALB/c mice vaccinated with B. abortus RB51. One selected positive clone (clone MCB68) contained an insert of 2.6 kb; nucleotide sequence analysis of this insert revealed two open reading frames (ORFs). The deduced amino acid sequences of the first and second ORFs had significant similarities with the YajC and SecD proteins, respectively, of several bacterial species. Both the YajC and SecD proteins were expressed in Escherichia coli as fusion proteins with maltose binding protein (MBP). In Western blots, sera from mice vaccinated with B. abortus RB51 recognized YajC but not SecD. Further Western blot analysis with purified recombinant YajC protein indicated that mice inoculated with B. abortus 19 or 2308 or B. melitensis RM1 also produced antibodies to YajC. In response to in vitro stimulation with recombinant MBP-YajC fusion protein, splenocytes from mice vaccinated with B. abortus RB51 were able to proliferate and produce gamma interferon but not interleukin-4. This study demonstrates, for the first time, the involvement of YajC protein in an immune response to an infectious agent.

Keywords: JOURNAL ARTICLE Amino Acid Sequence Animal Antigens, Bacterial/GENETICS/*IMMUNOLOGY Bacterial Proteins/GENETICS/*IMMUNOLOGY/*METABOLISM Base Sequence Brucella abortus/*IMMUNOLOGY Cloning, Molecular Comparative Study Escherichia coli/GENETICS Female Interferon Type II/BIOSYNTHESIS Mice Mice, Inbred BALB C Molecular Sequence Data Recombinant Fusion Proteins/IMMUNOLOGY Sequence Analysis, DNA Sequence Homology, Amino Acid Spleen/CYTOLOGY/IMMUNOLOGY Support, U.S. Gov't, Non-P.H.S. Th1 Cells/*IMMUNOLOGY Vaccination
990330
A9931066

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1999. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1999. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .