Critical level and detection limit: performance measures for PCR-based assays. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Critical level and detection limit: performance measures for PCR-based assays.

J Virol Methods. 1998 Oct;74(2):139-48. Unique Identifier : AIDSLINE MED/98450893
Markert-Hahn C; Berding C; Kuhn C; Boehringer Mannheim GmbH, Department of New Technologies LP-DNS,; Penzberg, Germany.; christine_markert-hahn@bmg.boehringer-mannheim.com

Abstract: In clinical diagnostic work, sensitivity and specificity are key assay features. In this note we introduce two clinically relevant statistical assay performance measures, the critical level (CL), and the detection limit (DL) for the PCR-based assay. To allow for easy access to these characteristics a Windows-based program has been developed. The application of CL and DL by means of examples is described.
Keywords: JOURNAL ARTICLE Hepatitis C/DIAGNOSIS Hepatitis C-Like Viruses/GENETICS/*ISOLATION & PURIF Human HIV-1/GENETICS/*ISOLATION & PURIF Reverse Transcriptase Polymerase Chain Reaction/*STANDARDS RNA, Viral/BLOOD Sensitivity and Specificity *SoftwareKWDjournalarticlehepatitisc/diagnosishepatitisc-likeviruses/genetics/KWDisolation&purifhumanhiv-1/genetics/KWDisolation&purifreversetranscriptasepolymerasechainreaction/KWDstandardsrna,viral/bloodsensitivityandspecificityKWDsoftware
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