Transgenic cattle produced by reverse-transcribed gene transfer in oocytes. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Transgenic cattle produced by reverse-transcribed gene transfer in oocytes.

Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14028-33. Unique Identifier : AIDSLINE MED/99045631
Chan AW; Homan EJ; Ballou LU; Burns JC; Bremel RD; Endocrinology-Reproductive Physiology Program, University of; Wisconsin, 1675 Observatory Drive, Madison, WI 53706, USA.

Abstract: A critical requirement for integration of retroviruses, other than HIV and possibly related lentiviruses, is the breakdown of the nuclear envelope during mitosis. Nuclear envelope breakdown occurs during mitotic M-phase, the envelope reforming immediately after cell division, thereby permitting the translocation of the retroviral preintegration complex into the nucleus and enabling integration to proceed. In the oocyte, during metaphase II (MII) of the second meiosis, the nuclear envelope is also absent and the oocyte remains in MII arrest for a much longer period of time compared with M-phase in a somatic cell. Pseudotyped replication-defective retroviral vector was injected into the perivitelline space of bovine oocytes during MII. We show that reverse-transcribed gene transfer can take place in an oocyte in MII arrest of meiosis, leading to production of offspring, the majority of which are transgenic. We discuss the implications of this mechanism both as a means of production of transgenic livestock and as a model for naturally occurring recursive transgenesis.
Keywords: JOURNAL ARTICLE Animal Animals, Transgenic Cattle Embryo Transfer Female *Gene Transfer Genetic Vectors Hepatitis B Surface Antigens/*GENETICS Kanamycin Kinase/*GENETICS Meiosis Metaphase *Moloney Leukemia Virus Oocytes/*PHYSIOLOGY Polymerase Chain Reaction/METHODS Pregnancy Repetitive Sequences, Nucleic Acid Sarcoma Viruses, Avian Support, Non-U.S. Gov't Transfection/METHODS Vesicular Stomatitis-Indiana Virus Zygote/PHYSIOLOGYKWDjournalarticleanimalanimals,transgeniccattleembryotransferfemaleKWDgenetransfergeneticvectorshepatitisbsurfaceantigens/KWDgeneticskanamycinkinase/KWDgeneticsmeiosismetaphaseKWDmoloneyleukemiavirusoocytes/KWDphysiologypolymerasechainreaction/methodspregnancyrepetitivesequences,nucleicacidsarcomaviruses,aviansupport,non-uKWDsKWDgov'ttransfection/methodsvesicularstomatitis-indianaviruszygote/physiology
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Copyright © 1999 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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