Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.
Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets.
Br J Haematol. 1999 Feb;104(2):220-9. Unique Identifier : AIDSLINE MED/99157988 Kowalska MA; Ratajczak J; Hoxie J; Brass LF; Gewirtz A; Poncz M; Ratajczak MZ; The Children's Hospital of Philadelphia, Pennsylvania 19104, USA.
Abstract:
Thrombocytopenia is a late complication of human immunodeficiency virus (HIV) infection. The chemokine receptor CXCR4 has been shown to be a co-receptor for lymphocyte-tropic HIV-1 strains. CXCR4 is also a natural receptor for the chemokine SDF-1. We have previously shown that CXCR1 and CXCR2 are present on megakaryocytes and platelets. Although interleukin-8 (IL-8) and other chemokines that bind to these two receptors do not activate platelets, they are able to inhibit megakaryocytopoiesis, presumably through these receptors. We therefore examined whether CXCR4 is present on developing and mature megakaryocytes and on platelets. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated the presence of CXCR4 message. Immature and mature alphaIIbbeta3+ megakaryocytes, and platelets were also positive for CXCR4 by flow cytometric studies using a CXCR4-specific antibody. We then tested whether SDF-1 can affect the biology of these cells. CD34+ cells and immature alphaIIbbeta3+ cells responded to SDF-1 as indicated by Ca2+ mobilization and chemotaxis. However, mature megakaryocytes failed to demonstrate either of these responses, in spite of their continued ability to bind 125I-SDF-1. Further, SDF-1 failed to inhibit megakaryocyte colony growth. Platelets bound 125I-SDF-1 with a K(D) similar to the affinity seen for CXCR4 on other cells, yet SDF-1 did not aggregate washed platelets nor augment aggregation by low-dose ADP or thrombin. SDF-1 also failed to stimulate Ca2+ mobilization, granular release or expression of P-selectin in platelets. Accordingly, although our studies demonstrate that CD34+ precursors, megakaryocytes and platelets all express CXCR4 and bind SDF-1, biological effects were only demonstrable of SDF-1 on CD34+ precursors. The potential biological implications of CXCR4 expression on maturing megakaryocytes and platelets in normal individuals and following HIV infection are discussed.
Keywords: JOURNAL ARTICLE Acquired Immunodeficiency Syndrome/METABOLISM Anti-HIV Agents/METABOLISM/*PHARMACOLOGY Blood Platelets/*METABOLISM Calcium/METABOLISM Chemokines, CXC/METABOLISM/*PHARMACOLOGY Flow Cytometry Hematopoietic Stem Cells/METABOLISM Human Megakaryocytes/*METABOLISM Neutrophils/METABOLISM Platelet Activation Receptors, CXCR4/*METABOLISM Reverse Transcriptase Polymerase Chain Reaction/METHODS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. 990630
A9961005
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Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.
