Development of a Western blot assay for detection of bovine immunodeficiency-like virus using capsid and transmembrane envelope proteins expressed from recombinant baculovirus.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Development of a Western blot assay for detection of bovine immunodeficiency-like virus using capsid and transmembrane envelope proteins expressed from recombinant baculovirus.

Clin Diagn Lab Immunol. 1999 Mar;6(2):168-72. Unique Identifier : AIDSLINE MED/99167659
Abed Y; St-Laurent G; Zhang H; Jacobs RM; Archambault D; Departement des Sciences Biologiques, Universite du Quebec a; Montreal, Montreal, Quebec, Canada.

Abstract: A 120-amino-acid polypeptide selected from the transmembrane protein region (tTM) and the major capsid protein p26 of bovine immunodeficiency-like virus (BIV) were expressed as fusion proteins from recombinant baculoviruses. The antigenic reactivity of both recombinant fusion proteins was confirmed by Western blot with bovine and rabbit antisera to BIV. BIV-negative bovine sera and animal sera positive for bovine syncytial virus and bovine leukemia virus failed to recognize the recombinant fusion proteins, thereby showing the specificity of the BIV Western blot. One hundred and five bovine serum samples were tested for the presence of anti-BIV antibodies by the recombinant protein-based Western blot and a reference Western blot assay using cell culture-derived virions as test antigens. There was a 100% concordance when the p26 fusion protein was used in the Western blot. However, the Western blot using the tTM fusion protein as its test antigen identified four BIV-positive bovine sera which had tested negative in both the p26 recombinant-protein-based and the reference Western blot assays. This resulted in the lower concordance of 96.2% between the tTM-protein-based and reference Western blot assays. The results of this study showed that the recombinant p26 and tTM proteins can be used as test antigens for the serodetection of BIV-infection in animals.

Keywords: JOURNAL ARTICLE Animal Antibodies, Viral Antibody Specificity Antigens, Viral/ANALYSIS/GENETICS/IMMUNOLOGY Baculoviridae/GENETICS Blotting, Western/*METHODS/STANDARDS Capsid/ANALYSIS/GENETICS/*IMMUNOLOGY Cattle Comparative Study Gene Expression Regulation, Viral Immunodeficiency Virus, Bovine/GENETICS/*IMMUNOLOGY/*ISOLATION & PURIF Plasmids Rabbits Recombinant Proteins/GENETICS/IMMUNOLOGY Sequence Analysis, DNA Support, Non-U.S. Gov't Viral Envelope Proteins/ANALYSIS/GENETICS/*IMMUNOLOGY
990630
A9960966

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1999. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1999. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .