Transverse 1H cross relaxation in 1H-15N correlated 1H CPMG experiments.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Transverse 1H cross relaxation in 1H-15N correlated 1H CPMG experiments.

J Magn Reson. 1999 Mar;137(1):289-92. Unique Identifier : AIDSLINE MED/99164243
Ishima R; Louis JM; Torchia DA; Molecular Structural Biology Unit, National Institute of Dental; and Craniofacial Research, Bethesda, Maryland, 20892-4326, USA.

Abstract: Transverse 1H cross relaxation was observed in Carr-Purcell-Meiboom-Gill (CPMG) experiments by recording 15N-1H correlated spectra of amides in HIV protease that was perdeuterated at nonexchangeable sites. Perdeuteration suppresses 1H-1H J coupling and improves spectral resolution and sensitivity. Measurements of cross-peak intensities, arising from cross relaxation, were made as a function of (i) Deltaf, the frequency difference between the spins, and (ii) tauCPMG, one-half of the duration between CPMG pi pulses. Cross peaks were observed when tauCPMG was less than 1/(2Deltaf), in agreement with theoretical calculations.

Keywords: JOURNAL ARTICLE Amides/*ANALYSIS Binding Sites Comparative Study HIV Protease/*CHEMISTRY Molecular Structure Nitrogen Isotopes/ANALYSIS Nuclear Magnetic Resonance/*METHODS Protons Spin Labels Support, U.S. Gov't, P.H.S. Urea/ANALOGS & DERIVATIVES/CHEMISTRY
990630
A9960957

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