Inhibition of nucleoside diphosphate kinase in rat liver mitochondria by added 3'-azido-3'-deoxythymidine. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Inhibition of nucleoside diphosphate kinase in rat liver mitochondria by added 3'-azido-3'-deoxythymidine.

FEBS Lett. 1999 Feb 12;444(2-3):291-5. Unique Identifier : AIDSLINE MED/99158064
Valenti D; Barile M; Quagliariello E; Passarella S; Dipartimento di Biochimica e Biologia Molecolare, Universita di; Bari, and Centro di Studi sui Mitocondri e Metabolismo; Energetico, C.N.R., Italy. d.valenti@biologia.uniba.it

Abstract: The effect of 3'-azido-3'-deoxythymidine on nucleoside diphosphate kinase of isolated rat liver mitochondria has been studied. This is done by monitoring the increase in the rate of oxygen uptake by nucleoside diphosphate (TDP, UDP, CDP or GDP) addition to mitochondria in state 4. It is shown that 3'-azido-3'-deoxythymidine inhibits the mitochondrial nucleoside diphosphate kinase in a competitive manner, with a Ki value of about 10 microM as measured for each tested nucleoside diphosphate. It is also shown that high concentrations of GDP prevent 3'-azido-3'-deoxythymidine inhibition of the nucleoside diphosphate kinase.
Keywords: JOURNAL ARTICLE Animal Atractyloside/ANALOGS & DERIVATIVES/PHARMACOLOGY Binding, Competitive Cytidine Diphosphate/PHARMACOKINETICS Enzyme Inhibitors/*PHARMACOLOGY Guanosine Diphosphate/PHARMACOKINETICS Kinetics Mitochondria, Liver/DRUG EFFECTS/*ENZYMOLOGY Nucleoside-Diphosphate Kinase/*ANTAGONISTS & INHIB Oligomycins/PHARMACOLOGY Oxygen Consumption/DRUG EFFECTS Rats Support, Non-U.S. Gov't Thymine Nucleotides/PHARMACOKINETICS Uridine Diphosphate/PHARMACOKINETICS Zidovudine/*PHARMACOLOGYKWDjournalarticleanimalatractyloside/analogs&derivatives/pharmacologybinding,competitivecytidinediphosphate/pharmacokineticsenzymeinhibitors/KWDpharmacologyguanosinediphosphate/pharmacokineticskineticsmitochondria,liver/drugeffects/KWDenzymologynucleoside-diphosphatekinase/KWDantagonists&inhiboligomycins/pharmacologyoxygenconsumption/drugeffectsratssupport,non-uKWDsKWDgov'tthyminenucleotides/pharmacokineticsuridinediphosphate/pharmacokineticszidovudine/KWDpharmacology
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