Antioxidant defenses influence HIV-1 replication and associated cytopathic effects. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Antioxidant defenses influence HIV-1 replication and associated cytopathic effects.

Free Radic Biol Med. 1998 Jun;24(9):1485-91. Unique Identifier : AIDSLINE MED/98302969
Sandstrom PA; Murray J; Folks TM; Diamond AM; HIV/Retrovirus Disease Branch, Division of AIDS, STD and TB; Laboratory Research, National Center for Infectious Disease,; Centers for Disease Control and Prevention, Atlanta, GA, USA.

Abstract: HIV-infected cells often exhibit reduced levels of antioxidant enzymes and thiols. To investigate the role of cellular antioxidant defenses in the progression of an acutely spreading HIV-1 infection, human Sup-T1 T cells were engineered to overexpress the selenium-dependent glutathione peroxidase, GSHPx-1. This enzyme represents a major cellular defense mechanism against toxicity associated with reactive oxygen species (ROS). T cells engineered to produce elevated GSHPx-1 activity displayed accelerated viral replication and associated cytopathic effects compared to control cells. Conversely, the inhibition of the synthesis of glutathione with buthione sulfoximine (BSO) resulted in the attenuation of viral replication in Sup-T1 cells. Similarly, exposure of human peripheral blood lymphocytes (PBLs) to low, nontoxic levels of BSO resulted in an approximately 80% decline in HIV-1 replication as indicated by Western blot analysis of viral proteins.
Keywords: JOURNAL ARTICLE Animal Antioxidants/*PHARMACOLOGY Benzene Derivatives/PHARMACOLOGY Buthionine Sulfoximine/PHARMACOLOGY Cattle Cell Line Clone Cells/DRUG EFFECTS Cytopathogenic Effect, Viral/*DRUG EFFECTS Glutathione/DRUG EFFECTS/METABOLISM Glutathione Peroxidase/GENETICS/PHYSIOLOGY Human HIV-1/CHEMISTRY/DRUG EFFECTS/*GROWTH & DEVELOPMENT Lymphocytes/DRUG EFFECTS/VIROLOGY Support, Non-U.S. Gov't T-Lymphocytes/CYTOLOGY/METABOLISM/VIROLOGY TransfectionKWDjournalarticleanimalantioxidants/KWDpharmacologybenzenederivatives/pharmacologybuthioninesulfoximine/pharmacologycattlecelllineclonecells/drugeffectscytopathogeniceffect,viral/KWDdrugeffectsglutathione/drugeffects/metabolismglutathioneperoxidase/genetics/physiologyhumanhiv-1/chemistry/drugeffects/KWDgrowth&developmentlymphocytes/drugeffects/virologysupport,non-uKWDsKWDgov'tt-lymphocytes/cytology/metabolism/virologytransfection
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