The SCID/Beige mouse as a model to investigate protection against Yersinia pestis.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

The SCID/Beige mouse as a model to investigate protection against Yersinia pestis.

FEMS Immunol Med Microbiol. 1999 Feb;23(2):107-13. Unique Identifier : AIDSLINE MED/99174525
Green M; Rogers D; Russell P; Stagg AJ; Bell DL; Eley SM; Titball RW; Williamson ED; DERA, Chemical and Biological Defense Sector, Salisbury,; Wiltshire, UK.

Abstract: In this study, we have shown that severe combined immunodeficient/beige mice reconstituted with hyperimmune Balb/c lymphocytes can be used as a model to demonstrate adoptive and passive protection against plague infection. Reconstitution of severe combined immunodeficient/beige mice was successful in nine out of ten mice as demonstrated by spleen colonisation and sustained circulating immunoglobulin titres. Furthermore, an increase in antibody titre was evident after a booster immunisation of reconstituted mice. Presence of circulating antibody correlated with protection against a systemic plague challenge and indicated that in reconstituted mice adoptive transfer of a functional immune system had occurred. The severe combined immunodeficient/beige mouse was also used to demonstrate passive protection against inhaled and systemic plague infection. The reconstituted severe combined immunodeficient/beige mouse model demonstrating protective immunity against plague will be further developed to identify the immune cell subsets responsible for this protection.

Keywords: JOURNAL ARTICLE *Adoptive Transfer Animal Antibodies, Bacterial/BIOSYNTHESIS/IMMUNOLOGY Antigens, Bacterial/IMMUNOLOGY *Disease Models, Animal Female Immune Sera/IMMUNOLOGY *Immunization, Passive Immunoglobulins/BIOSYNTHESIS/IMMUNOLOGY Liver/IMMUNOLOGY/MICROBIOLOGY Lung/IMMUNOLOGY/MICROBIOLOGY Lymphocytes/*IMMUNOLOGY Mice Mice, Inbred BALB C Mice, SCID Plague/*IMMUNOLOGY/MICROBIOLOGY/*PREVENTION & CONTROL Proteins/GENETICS Spleen/IMMUNOLOGY/MICROBIOLOGY Th2 Cells/IMMUNOLOGY Yersinia pestis/*IMMUNOLOGY
990730
A9971198

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