Single cell analysis of antigen-specific T cell responses in HIV-infected individuals. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Single cell analysis of antigen-specific T cell responses in HIV-infected individuals.

Cell Immunol. 1999 Mar 15;192(2):107-12. Unique Identifier : AIDSLINE MED/99189157
Byl B; Gerard M; Libin M; Clumeck N; Goldman M; Mascart F; Hopital Erasme, Universite Libre de Bruxelles, Brussels,; B-1070, Belgium.

Abstract: Antigen-specific and polyclonally induced T cell responses were analyzed in 10 HIV-infected individuals and in 14 controls by enumerating the numbers of tetanus toxoid (TT)-specific and phytohemagglutinin (PHA)-induced IFN-gamma-secreting cells (SC) and IL-4-SC using an enzyme-linked immunospot assay. Whereas the numbers of IFN-gamma-SC in HIV-infected patients were not different from those of the controls in response to an in vitro stimulation with PHA, they were significantly decreased in response to an in vitro stimulation with TT, both before and after a TT booster. Cell depletion experiments indicated that this difference was related to an impairment of CD4(+) T-cell-mediated TT-specific IFN-gamma secretion. Concerning IL-4, the numbers of both polyclonally induced IL-4-SC and TT-specific IL-4-SC were significantly lower in HIV-infected patients than in the controls. It is concluded that secretion of antigen-specific cytokines of both Th1 and Th2 types is depressed in HIV-infected patients. Copyright 1999 Academic Press.
Keywords: JOURNAL ARTICLE Adult Antigens/*IMMUNOLOGY Human HIV Infections/*IMMUNOLOGY IgG/BIOSYNTHESIS Interferon Type II/BIOSYNTHESIS Interleukin-4/BIOSYNTHESIS Middle Age Phytohemagglutinins/PHARMACOLOGY Support, Non-U.S. Gov't T-Lymphocytes/*IMMUNOLOGY Tetanus Toxoid/IMMUNOLOGYKWDjournalarticleadultantigens/KWDimmunologyhumanhivinfections/KWDimmunologyigg/biosynthesisinterferontypeii/biosynthesisinterleukin-4/biosynthesismiddleagephytohemagglutinins/pharmacologysupport,non-uKWDsKWDgov'tt-lymphocytes/KWDimmunologytetanustoxoid/immunology
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Copyright © 1999 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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