Transduction of CD34+ cells by a vesicular stomach virus protein G (VSV-G) pseudotyped HIV-1 vector. Stable gene expression in progeny cells, including dendritic cells.

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Transduction of CD34+ cells by a vesicular stomach virus protein G (VSV-G) pseudotyped HIV-1 vector. Stable gene expression in progeny cells, including dendritic cells.

J Hum Virol. 1998 Jul-Aug;1(5):346-52. Unique Identifier : AIDSLINE MED/99211166
Li X; Mukai T; Young D; Frankel S; Law P; Wong-Staal F; Department of Medicine, University of California, San Diego, La; Jolla, USA. fwongstaal@ucsd.edu

Abstract: OBJECTIVE: To use HIV-1 vectors to mediate stable gene transfer into hematopoietic stem/progenitor cells. STUDY DESIGN/METHODS: Purified human CD34+ cells were transduced with HIV-1 vectors pseudotyped with VSV-G and subjected to colony-forming assays and differentiation in liquid culture. Transduction was determined by DNA-polymerase chain reaction (PCR) for the transgene. GFP reporter gene expression and phenotypes of progeny cells were assessed by microscopy and flow cytometry. RESULTS: The HIV-1 vector transduced CD34+ cells with high efficiency. Transduction did not interfere with CD34+ cells differentiation in vitro. Transduced genes are expressed in different subsets of progeny cells, including those with normal dendritic cells (DC) morphology and phenotypes (HLADR+/CD1a+/CD86+/CD14-). CONCLUSIONS: We have demonstrated efficient transduction of hematopoietic progenitor cells by HIV-1 vectors. The transgenes are expressed in different subsets of progeny cells, which suggests stable integration. The generation of DCs stably expressing HIV antigens provides a new approach for vaccine development.

Keywords: JOURNAL ARTICLE Animal Antigens, CD34/*GENETICS Cell Differentiation COS Cells Dendritic Cells/CYTOLOGY/METABOLISM G-Proteins/*GENETICS Gene Expression/GENETICS Genes, Reporter/GENETICS Genes, Viral/GENETICS Genetic Vectors/ADMINISTRATION & DOSAGE Hematopoietic Stem Cells/*VIROLOGY Human HIV-1/*GENETICS/IMMUNOLOGY Leukocytes, Mononuclear Support, U.S. Gov't, P.H.S. Transfection/*METHODS Vesicular Stomatitis-Indiana Virus/*GENETICS
990730
A9971136

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