Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.
Activation of human herpesvirus 8 (HHV-8) thymidine kinase (TK) TATAA-less promoter by HHV-8 ORF50 gene product is SP1 dependent.
DNA Cell Biol. 1998 Sep;17(9):735-42. Unique Identifier : AIDSLINE MED/98449462 Zhang L; Chiu J; Lin JC; Centers for Disease Control and Prevention, Atlanta, GA 30333,; USA.
Abstract:
Human herpesvirus 8 (HHV-8) is a newly discovered virus closely associated with Kaposi's sarcoma and primary effusion lymphomas. When they occur in patients with AIDS, these B-cell lymphomas frequently harbor another human herpesvirus, Epstein-Barr virus (EBV). To determine the molecular mechanisms of the regulation of early gene expression by the immediate-early gene products of HHV-8 and to assess possible molecular interactions between HHV-8 and EBV, we studied the regulation of the HHV-8 thymidine kinase (TK) promoter in cell lines harboring either or both viruses. The constitutive chloramphenicol acetyltransferase (CAT) activity of the TK promoter was low in all six cell lines tested. A putative immediate-early gene product of HHV-8 ORF50, which is a homolog of EBV BRLF1, was cloned into an expression vector and tested for its transactivating capacity. In the presence of 12-O-tetradecanoyl-phorbol-13-acetate (TPA), the CAT activity of the TK promoter was increased 7- to 720-fold by cotransfection with the ORF50 clone in EBV-producing cell lines (Ramos/AW, P3HR-1, and BC-1) but not in EBV-negative cell lines (BCBL-1 and Ramos), nor in the latently EBV-infected cell line Raji. The TK promoter contains three consensus SP1- and two AP1-binding sites. In electrophoretic mobility shift assays, the cellular factor SP1, but not AP1, was found to bind specifically to the TK promoter. To determine whether the increased CAT activity resulted from the interaction of SP1 with the ORF50 gene product, we introduced mutations into two SP1-binding sites. Both mutated SP1 sites had reduced SP1-binding activity and greatly decreased TK promoter responsiveness to ORF50 transactivation, suggesting that upregulation of TK promoter by ORF50 is SP1 dependent.
Keywords: JOURNAL ARTICLE Base Sequence Cell Line Cloning, Molecular DNA, Viral/METABOLISM Genes, Immediate-Early/GENETICS Herpesvirus 4, Human/PHYSIOLOGY Herpesvirus, Kaposi Sarcoma-Associated/*GENETICS Human Immediate-Early Proteins/GENETICS Molecular Sequence Data Mutation/PHYSIOLOGY Promoter Regions (Genetics)/*GENETICS Protein Binding Recombinant Fusion Proteins Tetradecanoylphorbol Acetate/PHARMACOLOGY Thymidine Kinase/*GENETICS Trans-Activation (Genetics)/*GENETICS Transcription Factor, Sp1/METABOLISM Transcription Factors/GENETICS Viral Proteins/*GENETICS 990130
A9911079
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Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.
