IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.

J Clin Microbiol. 1998 Oct;36(10):3051-4. Unique Identifier : AIDSLINE MED/98411427
van Soolingen D; Bauer J; Ritacco V; Leao SC; Pavlik I; Vincent V; Rastogi N; Gori A; Bodmer T; Garzelli C; Garcia MJ; Diagnostic Laboratory of Infectious Diseases and Perinatal; Screening, National Institute of Public Health and the; Environment, 3720 BA Bilthoven, The Netherlands.; D.van.Soolingen@rivm.nl

Abstract: Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered.
Keywords: JOURNAL ARTICLE Animal AIDS-Related Opportunistic Infections/DIAGNOSIS/MICROBIOLOGY Databases, Factual Disease Reservoirs DNA Fingerprinting *DNA Transposable Elements Human Mycobacterium avium/*CLASSIFICATION/*GENETICS/ISOLATION & PURIF Polymerase Chain Reaction/METHODS *Polymorphism, Restriction Fragment Length Restriction Mapping Serotyping/METHODS Software Support, Non-U.S. Gov't Tuberculosis/*DIAGNOSIS/VETERINARYKWDjournalarticleanimalaids-relatedopportunisticinfections/diagnosis/microbiologydatabases,factualdiseasereservoirsdnafingerprintingKWDdnatransposableelementshumanmycobacteriumavium/KWDclassification/KWDgenetics/isolation&purifpolymerasechainreaction/methodsKWDpolymorphism,restrictionfragmentlengthrestrictionmappingserotyping/methodssoftwaresupport,non-uKWDsKWDgov'ttuberculosis/KWDdiagnosis/veterinary
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