Cell-associated viral RNA expression during acute infection with HIV type 1.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Cell-associated viral RNA expression during acute infection with HIV type 1.

AIDS Res Hum Retroviruses. 1998 Sep 1;14(13):1141-9. Unique Identifier : AIDSLINE MED/98407603
Riggs NL; Little SJ; Richman DD; Guatelli JC; Department of Medicine, University of California San Diego, La; Jolla 92093, USA.

Abstract: The mechanism of decline in viremia following acute infection with HIV is unknown. To characterize this process virologically, the expression of viral RNAs was analyzed in samples of peripheral blood mononuclear cells (PBMCs) from a patient who experienced a 100-fold decline in plasma viremia over a 13-day period prior to the initiation of antiretroviral therapy. Cell-associated viral RNA declined in association with the decline in plasma virus. During the initial 7 days of observation, plasma viremia declined more than 10-fold with no change in the ratio of unspliced to multiply spliced mRNAs. The efficiency of viral gene expression did not decline during the study period and varied from 380 to 2800 unspliced RNA copies per productively infected cell. Together, these data indicate no change in the relative proportion of cells in late- and early-stage gene expression during the initial decline and provide evidence against shortening of the viral replication cycle by immune surveillance. However, the prevalence of productively infected cells declined markedly during the 13 days of observation, from 1 in 250 to 1 in 25,000 PBMCs. These data are compatible with depletion of available target cells during the initial decline in viremia. As the level of plasma virus stabilized after 8 days of observation, the ratio of unspliced to multiply spliced mRNAs rose; this rise was due to a relatively greater decline in multiply spliced mRNA. These data suggest the possible onset of a blockade to new infection events (for example, by neutralizing antibody or chemokines), causing an increase in the relative proportion of cells in late-stage gene expression. They may also be explained, however, by the persistence of cell-associated virions together with the near disappearance of productively infected cells from the circulation.

Keywords: JOURNAL ARTICLE Acute Disease Human HIV Infections/*VIROLOGY *HIV-1 Leukocytes, Mononuclear/VIROLOGY Reverse Transcriptase Polymerase Chain Reaction RNA Splicing RNA, Viral/*BLOOD Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Time Factors Transcription, Genetic Viremia/VIROLOGY Virus Replication
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A9920921

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