Phenotypic changes in peripheral blood monocytes of cynomolgus monkeys acutely infected with simian immunodeficiency virus. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Phenotypic changes in peripheral blood monocytes of cynomolgus monkeys acutely infected with simian immunodeficiency virus.

Unique Identifier : AIDSLINE MED/98407607
Otani I; Akari H; Nam KH; Mori K; Suzuki E; Shibata H; Doi K; Terao K; Yosikawa Y; Tsukuba Primate Center, National Institute of Infectious; Diseases, Ibaraki, Japan.

Abstract: The quantitative and phenotypic changes of peripheral blood monocytes during the acute stage of simian immunodeficiency virus infection were investigated. We inoculated intravenously three cynomolgus monkeys (Macaca fascicularis) with 100 TCID50 of SIVmac239 and collected whole blood twice a week until 35 days postinoculation. We found that the relative number of monocytes in peripheral blood leukocytes significantly increased at 7-17 days postinoculation. This increase was concomitant with the peak of primary SIV antigenemia. To determine if the monocytes observed during the acute stage were phenotypically altered, they were periodically examined for the expression of surface markers (i.e., CD11b, CD14, CD16, CD29, D32, CD56, CD62L, CD64, CD80, and MHC-II-DR) by flow cytometry. The results showed that the expression levels of CD14 and CD56 on most of the monocytes were remarkably reduced at 7-17 days postinoculation, and a new subpopulation, CD14lowCD16+CD80+ monocytes, was clearly detected at 10 days postinoculation. These results indicate that the phenotypic alteration of peripheral blood monocytes occurs during the primary SIV infection.
Keywords: JOURNAL ARTICLE Acute Disease Animal Antigens, CD/*IMMUNOLOGY/*METABOLISM Flow Cytometry Macaca fascicularis Monocytes/*IMMUNOLOGY/*METABOLISM Phenotype Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGYKWDjournalarticleacutediseaseanimalantigens,cd/KWDimmunology/KWDmetabolismflowcytometrymacacafascicularismonocytes/KWDimmunology/KWDmetabolismphenotypesimianacquiredimmunodeficiencysyndrome/KWDimmunology
990228
A9920917

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