Primary intestinal epithelial cells can be infected with laboratory-adapted strain HIV type 1 NDK but not with clinical primary isolates. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Primary intestinal epithelial cells can be infected with laboratory-adapted strain HIV type 1 NDK but not with clinical primary isolates.

AIDS Res Hum Retroviruses. 1998 Sep 20;14(14):1235-8. Unique Identifier : AIDSLINE MED/98435935
Chenine AL; Matouskova E; Sanchez G; Reischig J; Pavlikova L; LeContel C; Chermann JC; Hirsch I; INSERM U322, Unite de Recherches sur les Retrovirus et maladies; associees, Marseille, France.

Abstract: Infectivities of HIV-1 primary isolates and laboratory-adapted strains were compared in primary fetal enterocytes and the colonic epithelial cell line HT29. Infection by two laboratory strains, HIV-1 NDK and HIV-1 NDK(A4), which were adapted on CEM and HT29 cells, respectively, produced significant amounts of virus in both target cell systems. Intestinal cells were resistant to infection with HIV-1 primary isolates regardless of their genetic subtype or SI/NSI phenotype. Biological properties of analyzed viruses rather than differences in cultivation system seem to be responsible for differences between these in vitro and ex vivo results.
Keywords: JOURNAL ARTICLE Cells, Cultured Epithelium/VIROLOGY Female Fetus Human HIV Infections/*VIROLOGY HIV-1/*ISOLATION & PURIF/PHYSIOLOGY/*PATHOGENICITY HT29 Cells Immunohistochemistry Intestinal Mucosa/*CYTOLOGY/*VIROLOGY Keratin/ANALYSIS Phenotype Pregnancy Support, Non-U.S. Gov'tKWDjournalarticlecells,culturedepithelium/virologyfemalefetushumanhivinfections/KWDvirologyhiv-1/KWDisolation&purif/physiology/KWDpathogenicityht29cellsimmunohistochemistryintestinalmucosa/KWDcytology/KWDvirologykeratin/analysisphenotypepregnancysupport,non-uKWDsKWDgov't
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A9920911

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