Replication-defective HIV as a vaccine candidate. NLM AIDSLINE Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

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Replication-defective HIV as a vaccine candidate.

AIDS Res Hum Retroviruses. 1998 Sep 20;14(14):1247-52. Unique Identifier : AIDSLINE MED/98435937
Tung FY; Rinaldo CR Jr; Montelaro RC; Department of Infectious Disease and Microbiology, University of; Pittsburgh, Pennsylvania 15261, USA.

Abstract: Live attenuated vaccines prepared from simian immunodeficiency virus (SIV) have provided the best protective immunity in challenge experiments. In animals vaccinated with attenuated SIV, immune responses may be elicited owing to endogenous expression of native SIV proteins and/or antigen presentation in the native replication site of virus. However, replication-competent viral vaccines raise safety concerns for clinical trials in humans. To ensure the safety and maintain the immunogenicity of a live, attenuated vaccine, we have developed a replication-defective HIV pseudotyped with vesicular stomatitis virus G protein (VSV-G). The polymerase gene of HIV was truncated to construct the replication-defective HIV. This pseudotyped HIV can infect many cell types, including human and simian cells, and undergoes only one round of replication. Furthermore, antibody immune response can be detected in mice immunized with VSV-G-pseudotyped replication-defective HIV.
Keywords: JOURNAL ARTICLE Animal AIDS Vaccines/GENETICS/*IMMUNOLOGY B-Lymphocytes Cell Line Gene Products, pol/GENETICS Human HIV Antibodies/BIOSYNTHESIS HIV Infections/IMMUNOLOGY/*PREVENTION & CONTROL HIV-1/GENETICS/*PHYSIOLOGY Immunization Mice Mice, Inbred BALB C Plasmids/GENETICS Support, U.S. Gov't, P.H.S. T-Lymphocytes, Cytotoxic Transduction, Genetic Vaccines, Attenuated/GENETICS/*IMMUNOLOGY Viral Envelope Proteins/*GENETICS *Virus ReplicationKWDjournalarticleanimalaidsvaccines/genetics/KWDimmunologyb-lymphocytescelllinegeneproducts,pol/geneticshumanhivantibodies/biosynthesishivinfections/immunology/KWDprevention&controlhiv-1/genetics/KWDphysiologyimmunizationmicemice,inbredbalbcplasmids/geneticssupport,uKWDsKWDgov't,pKWDhKWDsKWDt-lymphocytes,cytotoxictransduction,geneticvaccines,attenuated/genetics/KWDimmunologyviralenvelopeproteins/KWDgeneticsKWDvirusreplication
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