Molecular cloning and expression of rhesus macaque and sooty mangabey interleukin 16: biologic activity and effect on simian immunodeficiency virus infection and/or replication.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Molecular cloning and expression of rhesus macaque and sooty mangabey interleukin 16: biologic activity and effect on simian immunodeficiency virus infection and/or replication.

AIDS Res Hum Retroviruses. 1998 Oct 10;14(15):1323-8. Unique Identifier : AIDSLINE MED/99002722
Lee ME; Adams JW; Villinger F; Brar SS; Meadows M; Bucur SZ; Lackey DA 3rd; Brice GT; Cruikshank WW; Ansari AA; Hillyer CD; Department of Pathology and Laboratory Medicine and the Winship; Cancer Center, Emory University School of Medicine, Atlanta,; Georgia 30329, USA.

Abstract: Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through inhibition of HIV and SIV mRNA transcription. To evaluate its role further, we compared IL-16 cloned from disease-susceptible rhesus macaques and disease-resistant sooty mangabeys. Recombinant rhesus macaque (rr) IL-16 was compared with recombinant sooty mangabey (rm), human, and other nonhuman primate IL-16 sequences and evaluated for its ability to induce chemotaxis and inhibit the mixed lymphocyte response (MLR). Also, rrIL-16 and rmIL-16 were evaluated for suppression of SIVmac251, which replicates efficiently in T cells and monocyte/macrophages (dual tropic), and cloned SIVmac239, which replicates efficiently in T cells (T tropic). Sequence comparison of rrIL-16 and rmIL-16 with human IL-16 showed >97% amino acid identity. Biocharacterization of rrIL-16 revealed potent induction of chemotaxis (p < 0.05) and marked inhibition of MLR (73 +/- 0.6%,p < 0.05) in rhesus and human cell systems. Using rrIL-16 and rmIL-16, p27 antigen production from PBMCs infected with SIVmac251 was decreased up to 70% (p < 0.05 and p < 0.01, respectively). In similar cultures infected with SIVmac239, rrIL-16 and rmIL-16 reduced p27 levels by 96 and 100%, respectively. These data demonstrate the biologic and antiviral functionality of rrIL-16 and rmIL-16.

Keywords: JOURNAL ARTICLE Amino Acid Sequence Animal Cells, Cultured Cercocebus atys Chemotaxis, Leukocyte/DRUG EFFECTS Cloning, Molecular Human Interleukin-16/*GENETICS/PHARMACOLOGY Lymphocyte Culture Test, Mixed Macaca mulatta Macaca nemestrina Molecular Sequence Data Recombinant Proteins/GENETICS/PHARMACOLOGY Sequence Homology, Amino Acid Simian Acquired Immunodeficiency Syndrome/*DRUG THERAPY Support, U.S. Gov't, P.H.S. SIV/*DRUG EFFECTS T-Lymphocytes/CYTOLOGY/DRUG EFFECTS Virus Replication/DRUG EFFECTS
990228
A9920898

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1999. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1999. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .