Chemokine gene expression in human oral mucosa.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Chemokine gene expression in human oral mucosa.

Eur J Oral Sci. 1999 Aug;107(4):231-5. Unique Identifier : AIDSLINE MED/99396013
Zehnder M; Greenspan JS; Greenspan D; Bickel M; Department of Removable Prosthodontics, School of Dental; Medicine, University of Bern, Switzerland.

Abstract: In order to gain further understanding of the role of chemokines in healthy oral mucosa, we analyzed mRNA expression of the alpha (CXC)-family chemokines IL-8 and GROgamma as well as of the beta (CC)-family chemokines MIP-1alpha, MIP-1beta and MCP-1 in twenty young and healthy subjects with good oral hygiene. Twenty biopsies were taken from clinically healthy oral mucosa before surgical removal of impacted wisdom teeth. In addition, five biopsies from patients presenting with specific oral lesions were studied. RNA was purified, quantitated and utilized as substrate for competitive reverse transcription-polymerase chain reaction (RT-PCR). In healthy tissue, IL-8 and MCP-1 mRNA was constitutively expressed in all biopsies, whereas GROgamma, MIP-1alpha, and MIP-1beta were significantly lower. These findings suggest that IL8 and MCP-1 play a significant role in oral tissue homeostasis. The few samples from pathological conditions encourage exploring diseased tissue in more detail.

Keywords: JOURNAL ARTICLE Adolescence Adult Analysis of Variance Biopsy Chemokines, CC/*GENETICS Chemokines, CXC/*GENETICS Chemotactic Factors/GENETICS Female Fibroma/GENETICS/IMMUNOLOGY Gene Expression Regulation Gingival Neoplasms/GENETICS/IMMUNOLOGY Growth Inhibitors/GENETICS Growth Substances/GENETICS Hemostasis/GENETICS Human Interleukin-8/GENETICS Lichen Planus, Oral/GENETICS/IMMUNOLOGY Lymphoma, AIDS-Related/GENETICS/IMMUNOLOGY Macrophage Inflammatory Protein-1/GENETICS Male Monocyte Chemoattractant Protein-1/GENETICS Mouth Diseases/GENETICS/*IMMUNOLOGY Mouth Mucosa/*IMMUNOLOGY Mouth Neoplasms/GENETICS/IMMUNOLOGY Polymerase Chain Reaction RNA, Messenger/ANALYSIS/GENETICS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
991230
A99C1018

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