Cluster formation by protein kinase Ctheta during murine T cell activation: effect of age.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Cluster formation by protein kinase Ctheta during murine T cell activation: effect of age.

Cell Immunol. 1999 Jul 10;195(1):28-36. Unique Identifier : AIDSLINE MED/99365230
Yang D; Miller RA; Department of Pathology, University of Michigan, Ann Arbor,; Michigan, 48109-0940, USA.

Abstract: Protein kinase Ctheta; (PKCtheta;) is thought to play an important role in T cell activation, in that exposure of cloned T cells to antigen-presenting cells bearing agonist peptides, but not antagonist peptides, leads to clustering of PKCtheta; molecules in the section of the T cell plasma membrane that is in contact with the APCs. To see whether aging affects this PKCtheta; clustering reaction in mouse T lymphocytes, we used immunofluorescence staining and confocal microscopy to observe the localization of PKCtheta; in CD4 and CD8 T lymphocytes activated by coincubation with anti-CD3 hybridoma cells. Aging led to a twofold decline in the proportion of both CD4 and CD8 T cells in which PKCtheta; underwent cluster formation. This decrease with age was not due to differences in the number of cell conjugates formed, nor to kinetic differences of PKCtheta; clustering, nor to the accumulation of memory T cells in old mice. There were no effects of aging on the levels or kinase activity of PKCtheta; in murine T cells. Our data suggest alterations in the upstream signals that regulate PKCtheta; translocation. Copyright 1999 Academic Press.

Keywords: JOURNAL ARTICLE Aging/*IMMUNOLOGY Animal CD4-Positive T-Lymphocytes/*ENZYMOLOGY/IMMUNOLOGY CD8-Positive T-Lymphocytes/*ENZYMOLOGY/IMMUNOLOGY Enzyme Activation Hybridomas Isoenzymes/BIOSYNTHESIS/*METABOLISM Lymphocyte Transformation/*IMMUNOLOGY Male Mice Mice, Inbred C57BL Protein Kinase C/BIOSYNTHESIS/*METABOLISM Rats Spleen/IMMUNOLOGY Support, U.S. Gov't, P.H.S.
991230
A99C1002

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