Bovine insulin and porcine or human insulin prime distinct CD4(+) T cell subsets in C57BL/6 mice.

Important note: Information in this article was accurate in 1999. The state of the art may have changed since the publication date.

Bovine insulin and porcine or human insulin prime distinct CD4(+) T cell subsets in C57BL/6 mice.

Cell Immunol. 1999 Jul 10;195(1):66-74. Unique Identifier : AIDSLINE MED/99365234
Long SA; Zimecki M; Kapp JA; Department of Ophthalmology, Emory University, Atlanta, Georgia,; 30322, USA.

Abstract: H-2(b) mice produce insulin-specific antibody when injected with bovine but not porcine or human insulin. Nevertheless, CD4(+) T cells have been cloned from C57BL/6 mice primed with porcine, human, and bovine insulin. Here we tested the hypothesis that CD4(+) T cells from C57BL/6 mice primed with porcine or human insulin are functionally distinct from those primed with bovine insulin. Our results show that variants of insulin that stimulate antibody responses induced Th2 clones, whereas variants of insulin that fail to stimulate antibody induced Th0 clones. Th0 clones triggered delayed-type hypersensitivity (DTH) in adoptive recipients, whereas Th2 clones did not. Insulin variants that primed Th0 clones also directly primed for DTH responses, while variants that activated Th2 clones did not. Thus, induction of Th2 clones correlated with the ability of mice to make antibody responses to insulin while development of Th0 clones correlated with DTH responses and the failure to produce antibody. Copyright 1999 Academic Press.

Keywords: JOURNAL ARTICLE Adoptive Transfer Animal Cattle Cell Line Cytokines/BIOSYNTHESIS CD4-Positive T-Lymphocytes/CYTOLOGY/DRUG EFFECTS/*IMMUNOLOGY Hematopoietic Stem Cells/IMMUNOLOGY Human Hypersensitivity, Delayed Insulin/*IMMUNOLOGY/PHARMACOLOGY Mice Mice, Inbred C57BL Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Swine T-Lymphocyte Subsets/IMMUNOLOGY Th1 Cells/DRUG EFFECTS/IMMUNOLOGY Th2 Cells/DRUG EFFECTS/IMMUNOLOGY
991230
A99C1001

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