Human immunodeficiency virus type 1 Vif protein binds to the Pr55Gag precursor. NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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Human immunodeficiency virus type 1 Vif protein binds to the Pr55Gag precursor.

J Virol. 1997 Dec;71(12):9358-65. Unique Identifier : AIDSLINE MED/98037646
Bouyac M; Courcoul M; Bertoia G; Baudat Y; Gabuzda D; Blanc D; Chazal N; Boulanger P; Sire J; Vigne R; Spire B; INSERM U372, Marseille, France.

Abstract: The Vif protein of human immunodeficiency virus type 1 is required for productive replication in peripheral blood lymphocytes. Previous reports suggest that vif-deleted viruses are limited in replication because of a defect in the late steps of the virus life cycle. One of the remaining questions is to determine whether the functional role of Vif involves a specific interaction with virus core proteins. In this study, we demonstrate a direct interaction between Vif and the Pr55Gag precursor in vitro as well as in infected cells. No interaction is observed between Vif and the mature capsid protein. The Pr55Gag-Vif interaction is detected (i) in the glutathione S-transferase system, with in vitro-translated proteins demonstrating a critical role of the NC p7 domain of the Gag precursor; (ii) with proteins expressed in infected cells; and iii) by coimmunoprecipitation experiments. Deletion of the C-terminal 22 amino acids of Vif abolishes its interaction with the Pr55Gag precursor. Furthermore, point mutations in the C-terminal domain of Vif which have been previously shown to abolish virus infectivity and binding to cell membranes dramatically decrease the Gag-Vif interaction. These results suggest that the interaction between Vif and the pr55Gag precursor is a critical determinant of Vif function.
Keywords: *Gene Products, gag/METABOLISM *Gene Products, vif/METABOLISM *HIV-1/METABOLISM *Protein Precursors/METABOLISMKWDgeneproducts,gag/metabolismKWDgeneproducts,vif/metabolismKWDhiv-1/metabolismKWDproteinprecursors/metabolism
980330
M9831182

Copyright © 1998 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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