High-performance liquid chromatographic determination of ritonavir in human plasma, cerebrospinal fluid and saliva. NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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High-performance liquid chromatographic determination of ritonavir in human plasma, cerebrospinal fluid and saliva.

J Chromatogr B Biomed Sci Appl. 1998 Jan 23;705(1):119-26. Unique Identifier : AIDSLINE MED/98158443
Hoetelmans RM; van Essenberg M; Profijt M; Meenhorst PL; Mulder JW; Beijnen JH; Department of Pharmacy and Pharmacology, Slotervaart Hospital,; Amsterdam, The Netherlands.

Abstract: A simple, ion-pair high-performance liquid chromatographic method has been developed and validated for the quantitative determination of the HIV protease inhibitor ritonavir in human plasma, cerebrospinal fluid and saliva. Sample pretreatment consisted of precipitation of proteins with acetonitrile prior to high-performance liquid chromatography with ultraviolet detection at 239 nm. The method has been validated over the range of 50 ng/ml to 50 microg/ml with use of 100-microl volumes of sample. The currently described assay has been used successfully for the analysis of ritonavir in plasma, cerebrospinal fluid and saliva in HIV-1 infected patients.
Keywords: *HIV Protease Inhibitors/ANALYSIS *Ritonavir/ANALYSIS *Saliva/CHEMISTRYKWDhivproteaseinhibitors/analysisKWDritonavir/analysisKWDsaliva/chemistry
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