Cooperative interaction of ets-1 with USF-1 required for HIV-1 enhancer activity in T cells. NLM AIDSLINE Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.

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Cooperative interaction of ets-1 with USF-1 required for HIV-1 enhancer activity in T cells.

EMBO J. 1998 Mar 16;17(6):1728-39. Unique Identifier : AIDSLINE MED/98169389
Sieweke MH; Tekotte H; Jarosch U; Graf T; EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

Abstract: The distal enhancer region of the human immunodeficiency virus 1 HIV-1) long terminal repeat (LTR) is known to be essential for HIV replication and to contain immediately adjacent E-box and Ets binding sites. Based on a yeast one-hybrid screen we have identified the E-box binding protein USF-1 as a direct interaction partner of Ets-1 and found that the complex acts on this enhancer element. The binding surfaces of USF-1 and Ets-1 map to their DNA-binding domains and although these domains are highly conserved, the interaction is very selective within the respective protein family. USF-1 and Ets-1 synergize in specific DNA binding as well as in the transactivation of reporter constructs containing the enhancer element, and mutations of the individual binding sites dramatically reduce reporter activity in T cells. In addition, a dominant negative Ets-1 mutant inhibits both USF-1-mediated transactivation and the activity of the HIV-1 LTR in T cells. The inhibition is independent of Ets DNA-binding sites but requires the Ets binding surface on USF-1, highlighting the importance of the direct protein-protein interaction. Together these results indicate that the interaction between Ets-1 and USF-1 is required for full transcriptional activity of the HIV-1 LTR in T cells.
Keywords: *HIV Enhancer/GENETICS *HIV-1/GENETICS *Proto-Oncogene Proteins/METABOLISM *T-Lymphocytes/VIROLOGY *Transcription Factors/METABOLISM

KWDhivenhancer/geneticsKWDhiv-1/geneticsKWDproto-oncogeneproteins/metabolismKWDt-lymphocytes/virologyKWDtranscriptionfactors/metabolism
980730
M9871324

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