Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.
Modulatory function on autologous myeloid progenitor cells of clonal T-lymphocytes following autologous bone marrow transplantation.
Eur J Haematol. 1998 Mar;60(3):189-96. Unique Identifier : AIDSLINE MED/98208196 Hokland P; Carlsen I; Hokland M; Nielsen B; Department of Haematology, Aarhus University Hospital, Denmark.; hokland@gjallar.daimi.aau.dk
Abstract:
We have studied the regulatory capacity of clonal T-lymphocytes from patients undergoing autologous bone marrow transplantation ABMT) on the generation of CFU-GM from their harvest marrow preparations. To this end, T-lymphocytes from peripheral blood from 5 patients undergoing ABMT isolated 10 d before and 7, 14 and 28 d post-ABMT were placed in limiting dilution conditions 384 wells for each patient at each time point) and polyclonally stimulated. From more than 1600 wells with growth from the 5 patients, preparations from more than 900 wells could be expanded range between patients 33-452) and identified by immunophenotyping (IP) and flow cytometry (FCM) by their exclusive expression of CD4 or CD8. This was significantly fewer than seen in normal donors, especially so at d 7 and 14 post-ABMT The ratio between CD4+ and CD8+ clones varied between 0.6 and 2.8 median 1.3) and was significantly lower in the patients compared to normal donors (median 3.1; range 3.0-6.5). When the clonal T-cell preparations were co-cultured with autologous bone marrow cells obtained at the time of harvest and depleted for T-lymphocytes, the vast majority of both CD4+ and CD8+ clones exerted a clear enhancement on the CFU-GM growth with no relation to time of blood sampling in its the magnitude. Moreover, a trend seen in the normal donors towards CD4+ clones being more effective in this enhancement was not observed in ABMT patients. We conclude that clonal T-cells from ABMT patients, irrespective of their phenotype and time of isolation, exert an enhancement on the growth of autologous CFU-GM, which is equal to that seen in normal donors.
Keywords: *Bone Marrow Transplantation *T-Lymphocyte Subsets/PHYSIOLOGY
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Important note: Information in this article was accurate in 1998. The state of the art may have changed since the publication date.
